A Low-Cost Microfluidic Device For the On-Line Counting of Microparticle/Bacteria.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2024-12-03 DOI:10.1002/elps.202400061

Zhen-Yu Xun, Lv Liu, Bai-Chuan Zhang, Liang-Liang Fan, Lu-Wei Zhang, Hong Zhao, Sohail Iqbal, Rana Iqtidar Shakoor, Liang Zhao

{"title":"A Low-Cost Microfluidic Device For the On-Line Counting of Microparticle/Bacteria.","authors":"Zhen-Yu Xun, Lv Liu, Bai-Chuan Zhang, Liang-Liang Fan, Lu-Wei Zhang, Hong Zhao, Sohail Iqbal, Rana Iqtidar Shakoor, Liang Zhao","doi":"10.1002/elps.202400061","DOIUrl":null,"url":null,"abstract":"<p><p>On-line counting of the microparticle/bacteria in the liquid medium has great potential in the food safety and biomedical fields. A new low-cost microfluidic device is proposed for the on-line counting of the microparticles/bacteria in the liquid medium. The gradually contracted microchannel and the viscoelastic fluid are combined to achieve the efficient elastic focusing of the particle/bacteria, which significantly improves the counting accuracy by aligning all particles/bacteria in a single position at the center of the microchannel. A simple light resistance-based counting method is designed and integrated with the microchannel, where the low-cost elements including the laser pointer, convex lens, diaphragm, and photodiode, are used to build the optical path of detection. The influence of the flow rate, the particle size, the property of fluid, and the channel structure on the focusing of the particle/bacteria are investigated by the experiment, and the counting ability of the integrated microfluidic device is validated by using different-sized microparticles and the bifidobacterium. With its simple structure, low cost, easy operation, and high efficiency, this microfluidic device is suitable for the commercial applications, such as the on-line counting of the plastic microparticle in water or the colony-forming units (CFU) of bacteria in food.</p>","PeriodicalId":11596,"journal":{"name":"ELECTROPHORESIS","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ELECTROPHORESIS","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/elps.202400061","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

On-line counting of the microparticle/bacteria in the liquid medium has great potential in the food safety and biomedical fields. A new low-cost microfluidic device is proposed for the on-line counting of the microparticles/bacteria in the liquid medium. The gradually contracted microchannel and the viscoelastic fluid are combined to achieve the efficient elastic focusing of the particle/bacteria, which significantly improves the counting accuracy by aligning all particles/bacteria in a single position at the center of the microchannel. A simple light resistance-based counting method is designed and integrated with the microchannel, where the low-cost elements including the laser pointer, convex lens, diaphragm, and photodiode, are used to build the optical path of detection. The influence of the flow rate, the particle size, the property of fluid, and the channel structure on the focusing of the particle/bacteria are investigated by the experiment, and the counting ability of the integrated microfluidic device is validated by using different-sized microparticles and the bifidobacterium. With its simple structure, low cost, easy operation, and high efficiency, this microfluidic device is suitable for the commercial applications, such as the on-line counting of the plastic microparticle in water or the colony-forming units (CFU) of bacteria in food.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.