A Novel Method for Mitochondrial Membrane Potential Detection in Heart Tissue Following Ischemia-reperfusion in Mice.

IF 2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Current Medical Science Pub Date : 2024-12-01 Epub Date: 2024-12-04 DOI:10.1007/s11596-024-2956-1

Chao Yin, Chen-Xing Huang, Le Pan, Ke-Jia Jin, Ying Wang, Meng-Ying Cao, Hong Lin, Pan Gao, Na Li, Hui Gong, Yun-Zeng Zou

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引用次数: 0

Abstract

Objective: Myocardial ischemia-reperfusion (I/R) injury is associated with a significant reduction in the mitochondrial membrane potential (MMP, ΔΨm). Fluorescence-based assays are effective for labelling active mitochondria in living cells; their application in heart tissue, however, represents a challenge because of a low yield of viable cardiomyocytes after cardiac perfusion. This study aimed to examine a novel method for detecting the changes in the MMP of mouse heart tissue following I/R injury.

Methods: The I/R model was established, which was characterized by distinct ischemic area and apoptosis in heart tissue. The MMP was detected via a confocal microscope after the ascending aorta was clamped and the mitochondrial probe solution (containing Mito-Tracker Deep Red FM) was perfused from the apex via a peristaltic pump.

Results: This method enabled the distribution of the probe solution throughout the cardiac tissue via the coronary circulation. Fluorescence detection revealed that the MMP was profoundly reduced in both ischemic area and border area following I/R when compared with that in the sham group. There was no obvious difference in the MMP of the remote area between the I/R group and the sham group.

Conclusion: This study presents a novel method for detecting the MMP in heart tissue, and this method will facilitate the evaluation of changes in the MMP in different regions following I/R.

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小鼠心肌缺血再灌注后线粒体膜电位检测的新方法。

目的：心肌缺血再灌注（I/R）损伤与线粒体膜电位显著降低相关（MMP， ΔΨm）。荧光检测可有效标记活细胞中的活性线粒体；然而，由于心脏灌注后存活心肌细胞的产量低，它们在心脏组织中的应用代表了一个挑战。本研究旨在探讨一种检测I/R损伤后小鼠心脏组织MMP变化的新方法。方法：建立心肌缺血/再灌注模型，以心肌组织明显缺血区和凋亡为特征。夹紧升主动脉后，通过共聚焦显微镜检测MMP，线粒体探针溶液（含Mito-Tracker Deep Red FM）经蠕动泵从顶点灌注。结果：该方法使探针溶液通过冠状动脉循环分布于整个心脏组织。荧光检测显示，与假手术组相比，I/R后缺血区和边缘区MMP均明显减少。I/R组与假手术组之间远区MMP无明显差异。结论：本研究提出了一种检测心脏组织中MMP的新方法，该方法有助于评估I/R后不同区域MMP的变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Medical Science Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

4.70

自引率

0.00%

发文量

126

期刊介绍： Current Medical Science provides a forum for peer-reviewed papers in the medical sciences, to promote academic exchange between Chinese researchers and doctors and their foreign counterparts. The journal covers the subjects of biomedicine such as physiology, biochemistry, molecular biology, pharmacology, pathology and pathophysiology, etc., and clinical research, such as surgery, internal medicine, obstetrics and gynecology, pediatrics and otorhinolaryngology etc. The articles appearing in Current Medical Science are mainly in English, with a very small number of its papers in German, to pay tribute to its German founder. This journal is the only medical periodical in Western languages sponsored by an educational institution located in the central part of China.