Comparison of biocompatibility of epoxy resin and bioceramic-based root canal sealers.

IF 3.1 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Ahmet Demirhan Uygun, Serkan Şen, Sefa Çelik
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引用次数: 0

Abstract

Objectives: The purpose of this study is to compare the cytotoxicity of 2 bioceramic-based (Bioserra and AH Plus Bioceramic (Bio)) and 2 epoxy resin-based (Sealart and AH Plus) root canal sealers on the Saos-2 cell line.

Methods: The cytotoxicity of the root canal sealers was determined using the MTT metabolic activity assay. The mRNA expression levels of Bcl-2, Beclin1, Cas-3, IL-6, LC3, MMP-9 and TNF-α genes were assessed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and the expression of Beclin1 and LC3 proteins were assessed using western blot (WB) method. The canal sealers' total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were measured using photometric kits.

Results: AH Plus Bio significantly increased metabolic activity compared to the 2 resin-containing sealers, but there was no difference with Bioserra. Sealart was clearly the most cytotoxic group. QRT-PCR analysis revealed that the mRNA expressions of Bcl-2, Beclin1, Cas-3, IL-6, LC3, and MMP-9 in the Bioserra group, Bcl-2, Cas-3, and IL-6 in the Sealart group, Beclin1, Cas-3, IL-6, and MMP-9 in the AH Plus group, and Bcl2 in the AH Plus Bio group were stimulated. The OSI results showed no significant difference between the root canal sealer groups.

Conclusions: In the study, the best biocompatibility results in MTT, qRT-PCR, WB and OSI were in the AH Plus Bio group. Although Bioserra, the study's other bioceramic paste, was found to be biocompatible according to MTT and OSI measurements, it increased the expression of all genes in qRT-PCR except for TNF-α, and also increased LC3 protein levels in WB.

Clinical relevance: Since periradicular tissues can be directly affected by the materials used in root canal treatment, the sealers with high biocompatibility are being developed. In vitro studies examining the biocompatibility of newly produced root canal sealers are guiding for clinical success.

环氧树脂与生物陶瓷根管密封剂的生物相容性比较。
目的:本研究的目的是比较2种生物陶瓷基(Bioserra和AH Plus生物陶瓷(Bio))和2种环氧树脂基(Sealart和AH Plus)根管密封剂对Saos-2细胞系的细胞毒性。方法:采用MTT代谢活性法测定根管封闭剂的细胞毒性。采用定量逆转录-聚合酶链反应(qRT-PCR)检测Bcl-2、Beclin1、Cas-3、IL-6、LC3、MMP-9和TNF-α基因mRNA表达水平,采用western blot (WB)法检测Beclin1和LC3蛋白表达水平。采用光度测定试剂盒测定封堵者的总抗氧化状态(TAS)、总氧化状态(TOS)和氧化应激指数(OSI)。结果:与2种含树脂封口剂相比,AH Plus Bio显著提高了代谢活性,但与Bioserra没有差异。Sealart显然是细胞毒性最强的一组。QRT-PCR分析显示,Bioserra组Bcl-2、Beclin1、Cas-3、IL-6、LC3、MMP-9 mRNA表达受到刺激,Sealart组Bcl-2、Cas-3、IL-6、MMP-9 mRNA表达受到刺激,AH Plus组Beclin1、Cas-3、IL-6、MMP-9 mRNA表达受到刺激,AH Plus Bio组Bcl2 mRNA表达受到刺激。OSI结果显示根管封闭器组间无显著差异。结论:本研究中,AH + Bio组的MTT、qRT-PCR、WB和OSI的生物相容性最佳。虽然本研究的另一种生物陶瓷糊Bioserra根据MTT和OSI测量发现具有生物相容性,但它在qRT-PCR中增加了除TNF-α外的所有基因的表达,并且还增加了WB中LC3蛋白的水平。临床意义:由于根管治疗中使用的材料会直接影响根周组织,因此正在开发具有高生物相容性的密封剂。体外研究检测新生产的根管封闭剂的生物相容性对临床成功具有指导意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Clinical Oral Investigations
Clinical Oral Investigations 医学-牙科与口腔外科
CiteScore
6.30
自引率
5.90%
发文量
484
审稿时长
3 months
期刊介绍: The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.
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