Identification of ANS gene regulating fruit color in jujube (Ziziphus jujuba Mill. cv. Lingwuchangzao) and analysis of its expression under elevated temperature and drought stress.

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Botao Wang, Lei Zhang, Ting Zhang, Yaping Ma, Lihua Song
{"title":"Identification of ANS gene regulating fruit color in jujube (Ziziphus jujuba Mill. cv. Lingwuchangzao) and analysis of its expression under elevated temperature and drought stress.","authors":"Botao Wang, Lei Zhang, Ting Zhang, Yaping Ma, Lihua Song","doi":"10.1186/s12870-024-05837-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The anthocyanin synthase (ANS) gene is pivotal in plant anthocyanin biosynthesis, significantly influencing fruit coloration. Despite extensive on various studies, there remains a notable absence of research on the regulatory functions of the ANS gene in the 'Lingwuchangzao' variety under elevated temperature and drought stress.</p><p><strong>Results: </strong>In this study, 107 ZjANS genes were identified. Analyses of their physicochemical properties and gene structures revealed that most are localized in the cytosol and nucleus, with only seven ZjANS genes exhibiting basic characteristics. All genes contained the conserved domains 2OG-FeII_Oxy and DIOX-N. Chromosomal localization and Ka/Ks analysis indicated that these genes are distributed across 12 chromosomes, with 22 tandem gene pairs, suggesting purifying selection. Phylogenetic and synteny analysis demonstrated that ZjANS genes are evolutionarily conserved, forming collinear gene pairs with four species. Cis-acting element analysis identified elements related to anthocyanin synthesis and environmental stress, such as LTR and MBS. Predictions of protein interaction indicated that certain ZjANS proteins interact with MYB, CHI, and GA proteins, potentially involved in the anthocyanin synthesis pathway. Transcriptome and qRT-PCR analysis revealed that thirteen ZjANS genes positively respond to coloring in jujube under elevated temperature and drought stress, with ZjANS17 identified as particularly significant.</p><p><strong>Conclusion: </strong>This study identified 107 ZjANS genes and systematically analyzed their evolutionary conservation. Thirteen ZjANS genes were found to positively influence fruit coloring in 'Lingwuchangzao' under elevated temperature and drought stress. These findings provide a foundation for further investigations into ZjANS genes and the breeding new jujube varieties.</p>","PeriodicalId":9198,"journal":{"name":"BMC Plant Biology","volume":"24 1","pages":"1156"},"PeriodicalIF":4.3000,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610190/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12870-024-05837-4","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Background: The anthocyanin synthase (ANS) gene is pivotal in plant anthocyanin biosynthesis, significantly influencing fruit coloration. Despite extensive on various studies, there remains a notable absence of research on the regulatory functions of the ANS gene in the 'Lingwuchangzao' variety under elevated temperature and drought stress.

Results: In this study, 107 ZjANS genes were identified. Analyses of their physicochemical properties and gene structures revealed that most are localized in the cytosol and nucleus, with only seven ZjANS genes exhibiting basic characteristics. All genes contained the conserved domains 2OG-FeII_Oxy and DIOX-N. Chromosomal localization and Ka/Ks analysis indicated that these genes are distributed across 12 chromosomes, with 22 tandem gene pairs, suggesting purifying selection. Phylogenetic and synteny analysis demonstrated that ZjANS genes are evolutionarily conserved, forming collinear gene pairs with four species. Cis-acting element analysis identified elements related to anthocyanin synthesis and environmental stress, such as LTR and MBS. Predictions of protein interaction indicated that certain ZjANS proteins interact with MYB, CHI, and GA proteins, potentially involved in the anthocyanin synthesis pathway. Transcriptome and qRT-PCR analysis revealed that thirteen ZjANS genes positively respond to coloring in jujube under elevated temperature and drought stress, with ZjANS17 identified as particularly significant.

Conclusion: This study identified 107 ZjANS genes and systematically analyzed their evolutionary conservation. Thirteen ZjANS genes were found to positively influence fruit coloring in 'Lingwuchangzao' under elevated temperature and drought stress. These findings provide a foundation for further investigations into ZjANS genes and the breeding new jujube varieties.

红枣果实颜色调控基因ANS的鉴定。简历。lingwuchang藻)及其在高温和干旱胁迫下的表达分析。
背景:花青素合成酶(anthocyanin synthase, ANS)基因是植物花青素合成的关键基因,对果实的颜色有重要影响。尽管已有广泛的研究,但对‘灵武长藻’品种在高温干旱胁迫下ANS基因的调控功能的研究尚显不足。结果:共鉴定到107个ZjANS基因。理化性质和基因结构分析表明,ZjANS基因大部分定位于细胞质和细胞核,只有7个ZjANS基因具有基本特征。所有基因均含有保守结构域20g - fei_oxy和DIOX-N。染色体定位和Ka/Ks分析表明,这些基因分布在12条染色体上,有22对串联基因,可能是纯化选择。系统发育和同源性分析表明,ZjANS基因在进化上是保守的,与4个物种形成共线基因对。顺式作用元素分析鉴定出与花青素合成和环境胁迫相关的元素,如LTR和MBS。蛋白质相互作用的预测表明,某些ZjANS蛋白与MYB、CHI和GA蛋白相互作用,可能参与花青素合成途径。转录组分析和qRT-PCR分析结果显示,高温干旱胁迫下,13个zjans1基因对红枣着色有正向响应,其中ZjANS17基因的响应尤为显著。结论:本研究共鉴定出107个ZjANS基因,并对其进化保守性进行了系统分析。在高温干旱胁迫下,发现13个ZjANS基因对‘灵武长早’果实着色有正向影响。这些发现为进一步深入研究ZjANS基因和选育枣树新品种奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信