Establishment and Validation of Sandell-Kolthoff Method for Saliva Iodine Determination.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yue Su, Ming Li, Honglei Xie, Xin Hou, Meng Zhao, Jia Li, Haowen Pan, Zexu Zhang, Zongyu Yue, Wenjing Che, Lanchun Liu, Jiasen Cao, Peng Liu
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Abstract

Saliva iodine is a useful biochemical marker for evaluating iodine status and controlling iodine-related diseases. This study aims to establish and validate a simple, reliable, and low-cost Sandell-Kolthoff (S-K) method for the determination of iodine in saliva. The method was established by considering the digestion solution type, concentration, dosage, digestion time and temperature, the volume of saliva samples, glass tube size, dosage of arsenious acid solution and ammonium ceric sulfate solution, and warm bath time. Then, the effectiveness of the method was examined by the linearity of the standard curve, detection limit, precision, and accuracy. The 1.5 mL 1 Mol/L ammonium persulfate solution at 100 °C for 90 min, the 200 μL saliva sample, glass tubes of 15 mm × 120 mm, 2.5 mL arsenious acid solution, 0.3 mL ammonium ceric sulfate solution, and 15 min for warm bath was selected to determine saliva iodine. In terms of validation of the S-K method, the linearity method has the linear range of the standard curve from 0 to 300 μg/L and the coefficient of determination ≥ 0.999; the detection limit, when the volume of saliva samples was 0.2 mL, was 4.6 μg/L; for precision, the relative standard deviation was from 0.3 to 2.8%; and for accuracy, the total recovery rate ranged from 92.8 to 107.0%. This S-K method is easy to operate, has good precision and accuracy, and can be used as a method to determine saliva iodine. It has great potential practical value in disease prevention, clinical auxiliary diagnosis, and experimental research.

唾液碘测定的Sandell-Kolthoff法的建立与验证。
唾液碘是评价碘状态和控制碘相关疾病的重要生化指标。本研究旨在建立和验证一种简单、可靠、低成本的唾液中碘的Sandell-Kolthoff (S-K)法。考虑消解液种类、浓度、用量、消解时间、温度、唾液样品体积、玻璃管尺寸、亚砷酸溶液和硫酸铈铵溶液用量、温浴时间等因素,建立消解方法。通过标准曲线的线性度、检出限、精密度和准确度对方法的有效性进行了检验。选择1.5 mL 1 Mol/L过硫酸铵溶液,在100℃下放置90 min, 200 μL唾液样品,15 mm × 120 mm玻璃管,2.5 mL亚砷酸溶液,0.3 mL硫酸铈铵溶液,温浴15 min测定唾液碘。在S-K法的验证方面,线性方法的标准曲线线性范围为0 ~ 300 μg/L,测定系数≥0.999;唾液样品体积为0.2 mL时,检出限为4.6 μg/L;精密度的相对标准偏差为0.3 ~ 2.8%;准确度范围为92.8 ~ 107.0%。S-K法操作简便,精密度和准确度好,可作为测定唾液碘的方法。在疾病预防、临床辅助诊断、实验研究等方面具有潜在的实用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biological Trace Element Research
Biological Trace Element Research 生物-内分泌学与代谢
CiteScore
8.70
自引率
10.30%
发文量
459
审稿时长
2 months
期刊介绍: Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.
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阿拉丁
sodium chloride
阿拉丁
potassium iodate
阿拉丁
sodium hydroxide
阿拉丁
Ammonium persulfate (APS)
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