Nosocomial outbreak due to a novel sequence type of carbapenem-resistant Acinetobacter seifertii.

IF 3.8 3区医学 Q2 INFECTIOUS DISEASES

American journal of infection control Pub Date : 2024-11-29 DOI:10.1016/j.ajic.2024.11.019

Patricia Orlandi Barth, Dariane Castro Pereira, Gabriela Simões de Oliveira, Loriane Rita Konkewicz, Larissa Lutz, William Latosinski Matos, Mariana Preussler Mott, Caroline Collioni Constante, Camila Morschbacher Wilhelm, Laura Czekster Antochevis, Rodrigo Minuto Paiva, Kellen Figueira Tragnago, Afonso Luis Barth, Andreza Francisco Martins

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引用次数: 0

Abstract

Background: Acinetobacter seifertii, a member of A baumannii-calcoaceticus complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant A seifertii among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.

Methods: All patients with carbapenem-resistant A seifertii diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (bla_IMP, bla_VIM, bla_NDM, bla_KPC, bla_GES, bla_OXA-48-like, and bla_OXA-23) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.

Results: Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the bla_NDM-1 gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the bla_NDM-1 was carried by the same plasmid type.

Discussion: Our study has identified a novel strain of A seifertii carrying bla_NDM-1 responsible for the outbreak, indicating its emergence in the institution.

Conclusions: This is the first report of carbapenem-resistant A seifertii ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant A baumannii, which is crucial in outbreak scenarios.

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来源期刊

American journal of infection control 医学-传染病学

CiteScore

7.40

自引率

4.10%

发文量

479

审稿时长

24 days

期刊介绍： AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)