Nosocomial outbreak due to a novel sequence type of carbapenem-resistant Acinetobacter seifertii

IF 3.8 3区医学 Q2 INFECTIOUS DISEASES

American journal of infection control Pub Date : 2024-11-29 DOI:10.1016/j.ajic.2024.11.019

Patricia Orlandi Barth MSc , Dariane Castro Pereira PhD , Gabriela Simões de Oliveira MSc , Loriane Rita Konkewicz MSc , Larissa Lutz PhD , William Latosinski Matos , Mariana Preussler Mott PhD , Caroline Collioni Constante MSc , Camila Morschbacher Wilhelm PhD , Laura Czekster Antochevis MSc , Rodrigo Minuto Paiva PhD , Kellen Figueira Tragnago , Afonso Luis Barth PhD , Andreza Francisco Martins PhD

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引用次数: 0

Abstract

Background

Acinetobacter seifertii, a member of A baumannii-calcoaceticus complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant A seifertii among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.

Methods

All patients with carbapenem-resistant A seifertii diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (bla_IMP, bla_VIM, bla_NDM, bla_KPC, bla_GES, bla_OXA-48-like, and bla_OXA-23) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.

Results

Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the bla_NDM-1 gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the bla_NDM-1 was carried by the same plasmid type.

Discussion

Our study has identified a novel strain of A seifertii carrying bla_NDM-1 responsible for the outbreak, indicating its emergence in the institution.

Conclusions

This is the first report of carbapenem-resistant A seifertii ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant A baumannii, which is crucial in outbreak scenarios.

查看原文本刊更多论文

一种新型序列型耐碳青霉烯不动杆菌引起的医院暴发。

背景：作为鲍曼不动杆菌-钙酸杆菌复合体的一员，由于耐药基因的存在，可被认为是一种值得关注的病原体。本研究的目的是描述一家三级医院新生儿重症监护病房（NICU）收治的新生儿中碳青霉烯类耐药单胞杆菌（CRAS）的爆发。方法：纳入2023年6月至2023年10月期间所有诊断为CRAS并入住NICU的患者。采用qPCR检测碳青霉烯酶基因blaIMP、blaVIM、blaNDM、blaKPC、blaGES、blaoxa -48 like和blaOXA-23的表达。全基因组测序（WGS）采用MiSeq （Illumina）和MinION （Nanopore）平台进行。采用FTIR （IR Biotyper®）进行微生物菌株分型。结果：11例患者受到感染，NICU实施了一系列措施，降低了传播风险。所有分离株均具有blaNDM-1基因，并通过IR Biotyper将其归为同一簇。WGS结果显示，分离株属于ST2712， blaNDM-1由同一质粒型携带。讨论：我们的研究已经确定了一种携带blaNDM-1的新型seifertii菌株，这表明它在该机构出现。结论：本文为首次报道耐碳青霉烯类单胞杆菌ST2712。使用WGS进行基因组监测有助于了解螃蟹的传播情况，这在暴发情况下至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of infection control 医学-传染病学

CiteScore

7.40

自引率

4.10%

发文量

479

审稿时长

24 days

期刊介绍： AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)