Nosocomial outbreak due to a novel sequence type of carbapenem-resistant Acinetobacter seifertii

IF 3.8 3区 医学 Q2 INFECTIOUS DISEASES
Patricia Orlandi Barth MSc , Dariane Castro Pereira PhD , Gabriela Simões de Oliveira MSc , Loriane Rita Konkewicz MSc , Larissa Lutz PhD , William Latosinski Matos , Mariana Preussler Mott PhD , Caroline Collioni Constante MSc , Camila Morschbacher Wilhelm PhD , Laura Czekster Antochevis MSc , Rodrigo Minuto Paiva PhD , Kellen Figueira Tragnago , Afonso Luis Barth PhD , Andreza Francisco Martins PhD
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引用次数: 0

Abstract

Background

Acinetobacter seifertii, a member of A baumannii-calcoaceticus complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant A seifertii among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.

Methods

All patients with carbapenem-resistant A seifertii diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (blaIMP, blaVIM, blaNDM, blaKPC, blaGES, blaOXA-48-like, and blaOXA-23) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.

Results

Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the blaNDM-1 gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the blaNDM-1 was carried by the same plasmid type.

Discussion

Our study has identified a novel strain of A seifertii carrying blaNDM-1 responsible for the outbreak, indicating its emergence in the institution.

Conclusions

This is the first report of carbapenem-resistant A seifertii ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant A baumannii, which is crucial in outbreak scenarios.
一种新型序列型耐碳青霉烯不动杆菌引起的医院暴发。
背景:作为鲍曼不动杆菌-钙酸杆菌复合体的一员,由于耐药基因的存在,可被认为是一种值得关注的病原体。本研究的目的是描述一家三级医院新生儿重症监护病房(NICU)收治的新生儿中碳青霉烯类耐药单胞杆菌(CRAS)的爆发。方法:纳入2023年6月至2023年10月期间所有诊断为CRAS并入住NICU的患者。采用qPCR检测碳青霉烯酶基因blaIMP、blaVIM、blaNDM、blaKPC、blaGES、blaoxa -48 like和blaOXA-23的表达。全基因组测序(WGS)采用MiSeq (Illumina)和MinION (Nanopore)平台进行。采用FTIR (IR Biotyper®)进行微生物菌株分型。结果:11例患者受到感染,NICU实施了一系列措施,降低了传播风险。所有分离株均具有blaNDM-1基因,并通过IR Biotyper将其归为同一簇。WGS结果显示,分离株属于ST2712, blaNDM-1由同一质粒型携带。讨论:我们的研究已经确定了一种携带blaNDM-1的新型seifertii菌株,这表明它在该机构出现。结论:本文为首次报道耐碳青霉烯类单胞杆菌ST2712。使用WGS进行基因组监测有助于了解螃蟹的传播情况,这在暴发情况下至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.40
自引率
4.10%
发文量
479
审稿时长
24 days
期刊介绍: AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)
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