{"title":"Nosocomial outbreak due to a novel sequence type of carbapenem-resistant Acinetobacter seifertii","authors":"Patricia Orlandi Barth MSc , Dariane Castro Pereira PhD , Gabriela Simões de Oliveira MSc , Loriane Rita Konkewicz MSc , Larissa Lutz PhD , William Latosinski Matos , Mariana Preussler Mott PhD , Caroline Collioni Constante MSc , Camila Morschbacher Wilhelm PhD , Laura Czekster Antochevis MSc , Rodrigo Minuto Paiva PhD , Kellen Figueira Tragnago , Afonso Luis Barth PhD , Andreza Francisco Martins PhD","doi":"10.1016/j.ajic.2024.11.019","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><em>Acinetobacter seifertii</em>, a member of <em>A baumannii-calcoaceticus</em> complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant <em>A seifertii</em> among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.</div></div><div><h3>Methods</h3><div>All patients with carbapenem-resistant <em>A seifertii</em> diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (<em>bla</em><sub>IMP</sub>, <em>bla</em><sub>VIM</sub>, <em>bla</em><sub>NDM</sub>, <em>bla</em><sub>KPC</sub>, <em>bla</em><sub>GES</sub>, <em>bla</em><sub>OXA-48-like</sub>, and <em>bla</em><sub>OXA-23</sub>) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.</div></div><div><h3>Results</h3><div>Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the <em>bla</em><sub>NDM-1</sub> gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the <em>bla</em><sub>NDM-1</sub> was carried by the same plasmid type.</div></div><div><h3>Discussion</h3><div>Our study has identified a novel strain of <em>A seifertii</em> carrying <em>bla</em><sub>NDM-1</sub> responsible for the outbreak, indicating its emergence in the institution.</div></div><div><h3>Conclusions</h3><div>This is the first report of carbapenem-resistant <em>A seifertii</em> ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant <em>A baumannii</em>, which is crucial in outbreak scenarios.</div></div>","PeriodicalId":7621,"journal":{"name":"American journal of infection control","volume":"53 4","pages":"Pages 473-478"},"PeriodicalIF":3.8000,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of infection control","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0196655324008599","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background
Acinetobacter seifertii, a member of A baumannii-calcoaceticus complex, can be considered a pathogen of concern due to the presence of resistance genes. The aim of the study was to describe an outbreak of carbapenem-resistant A seifertii among neonates admitted to the neonatal intensive care unit (NICU) at a tertiary care hospital.
Methods
All patients with carbapenem-resistant A seifertii diagnosed and admitted to the NICU from June 2023 to October 2023 were included. The presence of carbapenemase genes (blaIMP, blaVIM, blaNDM, blaKPC, blaGES, blaOXA-48-like, and blaOXA-23) was investigated by qPCR. Whole-genome sequencing (WGS) was performed by MiSeq (Illumina) and MinION (Nanopore) platforms. Fourier-transform infrared spectroscopy (IR Biotyper) was applied for microbial strain typing.
Results
Eleven patients were affected and a set of measures were implemented at NICU to reduce the risk of transmission. The isolates exhibited identical resistance patterns; additionally, all isolates presented the blaNDM-1 gene and were grouped in the same cluster by IR Biotyper. The WGS revealed that the isolates belonged to a novel ST assigned as ST2712, and the blaNDM-1 was carried by the same plasmid type.
Discussion
Our study has identified a novel strain of A seifertii carrying blaNDM-1 responsible for the outbreak, indicating its emergence in the institution.
Conclusions
This is the first report of carbapenem-resistant A seifertii ST2712. The use of WGS for genomic surveillance allowed understanding the dissemination of Carbapenem-resistant A baumannii, which is crucial in outbreak scenarios.
期刊介绍:
AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)