Caloric restriction mimetics: Pinostilbene versus resveratrol regarding SIRT1 and SIRT6 interaction.

IF 2.5 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Anca Ungurianu, Denisa Margină, Dragoș P Mihai, Alina C Nicolae, Cristina M Drăgoi, Daniela Grădinaru, Anca Zanfirescu
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引用次数: 0

Abstract

Purpose: Caloric restriction (CR), the permanent or periodic reduction of caloric intake, is a dietary strategy that promotes longevity and healthspan, yielding multiple beneficial effects, such as improved insulin sensitivity and mitochondrial function, decreased body weight, and mitigation of cardiometabolic risk factors. The purpose of our study was the in silico and in vitro assessment of the effects exerted by pinostilbene on SIRT1 and SIRT6 compared to those of resveratrol, a known activator of these enzymes.

Materials and methods: Molecular docking was carried out to determine the interactions with SIRT1 and SIRT6 and, further, the effect of pinostilbene on their activity was tested in vitro to evaluate if it parallels resveratrol's effects regarding SIRT activation.

Results: Molecular docking indicates that resveratrol and pinostilbene bind similarly to SIRT6, while pinostilbene may be able to activate SIRT1 more efficiently than resveratrol. In vitro activity assays showed that while both resveratrol and pinostilbene activate SIRT1 and SIRT6, the concentration-dependent effects differ. For resveratrol, a greater effect was observed at the medium concentration (25 ​μM), whereas pinostilbene showed a more pronounced activation at the lowest concentration (5 ​μM).

Conclusions: Our results offer a glimpse into the structural features and interactions of pinostilbene and resveratrol with SIRT1 and SIRT6, contributing to understanding their potential roles in various cellular processes regulated by SIRT.

热量限制模拟:蒎烯与白藜芦醇对SIRT1和SIRT6相互作用的影响。
目的:热量限制(CR),即永久或定期减少热量摄入,是一种促进长寿和健康的饮食策略,可产生多种有益效果,如改善胰岛素敏感性和线粒体功能,降低体重,减轻心脏代谢危险因素。我们研究的目的是在体内和体外评估pinostilbene对SIRT1和SIRT6的影响,并与白藜芦醇(一种已知的这些酶的激活剂)进行比较。材料与方法:通过分子对接确定与SIRT1和SIRT6的相互作用,并进一步在体外测试蒎烯对其活性的影响,以评估其是否与白藜芦醇对SIRT激活的作用相似。结果:分子对接表明,白藜芦醇和蒎烯与SIRT6的结合相似,而蒎烯可能比白藜芦醇更有效地激活SIRT1。体外活性分析表明,尽管白藜芦醇和蒎烯都能激活SIRT1和SIRT6,但其浓度依赖性不同。对于白藜芦醇,在中等浓度(25 μM)下的活化效果更明显,而蒎烯在最低浓度(5 μM)下的活化效果更明显。结论:我们的研究结果揭示了蒎烯二苯乙烯和白藜芦醇与SIRT1和SIRT6的结构特征和相互作用,有助于了解它们在SIRT调控的各种细胞过程中的潜在作用。
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来源期刊
Advances in medical sciences
Advances in medical sciences 医学-医学:研究与实验
CiteScore
5.00
自引率
0.00%
发文量
53
审稿时长
25 days
期刊介绍: Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines. The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments. Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines. The journal welcomes submissions from the following disciplines: General and internal medicine, Cancer research, Genetics, Endocrinology, Gastroenterology, Cardiology and Cardiovascular Medicine, Immunology and Allergy, Pathology and Forensic Medicine, Cell and molecular Biology, Haematology, Biochemistry, Clinical and Experimental Pathology.
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