Structural Evolution of an Optimized Highly Interconnected Hierarchical Porous Mg Scaffold under Dynamic Flow Challenges.

Abstract

The development of Mg and its alloys as bone screws has garnered significant attention due to their exceptional biocompatibility and unique biodegradability. Notably, the controlled release of Mg²⁺ ions during degradation can positively influence bone fracture healing. The advantages of Mg raise appeal for application in bone tissue engineering. However, porous Mg scaffolds, while offering high surface areas, face challenges in maintaining slow degradation rates and preserving interconnectivity, which are crucial features for tissue ingrowth. To address these issues, this study introduces a highly interconnected hierarchical porous Mg scaffold and investigates its degradation behavior within a bioreactor, simulating body fluid flow rates to mimic the in vivo degradation performance at different implantation sites. The focus lies on elucidating the evolution of the porous structure, particularly the impact of degradation behavior on scaffold interconnectivity. Our findings reveal that the initial high interconnectivity of the scaffold is significantly influenced by the flow rate. The dynamic fluid flow modulates the transport of degradation byproducts and the deposition patterns. At lower flow rates, Mg²⁺ ions accumulate within pores, leading to the formation of substantial deposits that directly reduce porosity. Specifically, after 42 days, porosities decreased to 68.80 ± 2.31, 58.52 ± 2.53, and 41.25 ± 2.82% at flow rates of 2.0, 1.0, and 0.5 mL/min, respectively. This porosity reduction and pore space occlusion by deposits sequentially hinder the interconnectivity. The magnitude of decreased porosity could be used to evaluate the ability of the microarchitecture to maintain scaffold interconnectivity. Meanwhile, the long-term degradation deposition behavior of the highly interconnected hierarchical porous Mg scaffold potentially revealed the structural integrity loss from the original design to its in vivo degraded structure at different body fluid flow rates. The present work might bring valuable insight into the design of pore strut and interconnectivity characterization methods for the progress of a high-performance tissue engineering scaffold.