Engineering Interference-Removal and Target-Response Cascade Zones on Wood Channels for Straightforward Detection of Uric Acid in Saliva

Abstract

The detection of biomarkers is crucial for assessing disease status and progression. Uric acid (UA), a common biomarker in body fluids, plays an important role in the diagnosis and monitoring of conditions such as hyperuricemia, chronic kidney disease, and cardiovascular disease. However, the low concentration of UA in non-invasive body fluids, combined with numerous interfering substances, makes its detection challenging. Therefore, there is a pressing need to develop advanced sensor platforms that exhibit both high sensitivity and excellent specificity for accurate monitoring of UA levels in various body fluids. In this study, an electrochemical sensing system is developed by integrating a cascade interference-removal zone and a target-response zone on a wooden channel membrane (CM) for the pretreatment-free detection of UA in body fluids. In this design, cysteine-doped ZIF-67(Co) nanocrystals, a mimic with multi-enzyme activity, are modified in the interference-removal zone. The target-response zone on the other side of the CM is contacted with a solution containing Fe³⁺ and [Fe(CN)₆^]3– ions. Using saliva as a proof-of-concept, the interference species, including ascorbic acid (AA), dopamine (DA), and glutathione (GSH), are oxidized and removed when passing through the interference-removal zone, while UA reaches the target-response zone and triggers the growth of Prussian blue (PB) on site. Utilizing the peroxidase-like activity of PB, UA concentration is directly determined based on changes in transmembrane ion currents. The resulting sensing system exhibits higher sensitivity than commercial UA meters and demonstrates straightforward, continuous, and non-invasive monitoring of UA in saliva after consuming purine-rich foods. The technology provides a simple, reliable and low-cost device for sensing UA in complex biological matrices. Moreover, the CM based sensing device also provides the benefit of being incineratable and biodegradable, reducing medical and electronic waste, making it eco-friendly for daily diagnostics.