Enhanced Vitamin B12 Detection Using a CxNixOyFeyOx+3 Bimetal-Organic Framework: A Comprehensive Electrochemical Study

IF 5.5 3区 材料科学 Q1 ELECTROCHEMISTRY
Sivalingam Gopi, Annadurai Thamilselvan, Moon Il Kim, Kyusik Yun
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引用次数: 0

Abstract

The functions of neurons and blood cells are aided by vitamin B12. Serum vitamin B12 levels are picomolar, and their deficiencies may be remedied using vitamin B12 tablets. Consequently, sensitive and selective vitamin B12 detectors that can be used at the point-of-care for food, pharmaceutical, and biological samples are urgently needed. In this study, we present the synthesis and carbonization of a Ni- and Fe-based bimetallic organic framework for the electrochemical sensing of vitamin B12. The crystalline nature and functional groups of the material were analyzed using X-ray diffraction and Fourier transform infrared (FT-IR) spectroscopy, and the oxidation states within the framework were determined using X-ray photoelectron analysis. Field-emission scanning electron microscopy revealed a uniform spherical morphology with particle sizes ranging from 2 to 10 nm. Electrochemical analysis was performed to detect vitamin B12 by cyclic voltammetry (CV) on a screen-printed carbon electrode modified with CxNixOyFeyOx+3. CV analysis revealed that the fabricated electrode exhibits excellent redox peaks at approximately 0.0245 and -0.514 V with a low detection limit of 49 nM for vitamin B12 and a wide linear detection range up to 200 µM. Interference studies confirmed the selectivity of the sensor with minimal impact from common biomolecules and inorganic ions. The sensor demonstrated excellent repeatability (RDS = 3.5%), reproducibility, and long-term stability, retaining 94% of its initial current response for over 20 d. The real sample analysis of commercial vitamin B complex tablets demonstrated a recovery rate of 98.59%, highlighting the practical applicability of the sensor in pharmaceutical analysis.

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来源期刊
Electrochimica Acta
Electrochimica Acta 工程技术-电化学
CiteScore
11.30
自引率
6.10%
发文量
1634
审稿时长
41 days
期刊介绍: Electrochimica Acta is an international journal. It is intended for the publication of both original work and reviews in the field of electrochemistry. Electrochemistry should be interpreted to mean any of the research fields covered by the Divisions of the International Society of Electrochemistry listed below, as well as emerging scientific domains covered by ISE New Topics Committee.
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