Selective colorimetric detection of carbosulfan based on its hydrolysis behavior and Ti3C2/AuPt nanozyme

IF 5.7 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Shuxian Dai , Mengting Hu , Wen Zhang , Zhen Lei
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引用次数: 0

Abstract

Background

Carbosulfan (CBS) is a widely used carbamate pesticide in agricultural production, its easy decomposition into hypertoxic carbofuran poses serious threats to human health and food safety. Therefore, sensitive and accurate detection of CBS is of significant importance. Conventional chromatography-based techniques require expensive instruments and complicated sample pretreatment, limiting their application for fast detection. Current electrochemical and colorimetric methods for detection of pesticides based on the cascade catalytic reactions between acetylcholinesterase (AChE) and nanozymes, which exhibit inferior selectivity. Hence, selective, sensitive and fast detection of CBS is still challenging.

Results

In this work, an AChE-free colorimetric method was proposed for selective detection of CBS based on its unique hydrolysis behavior and nanozyme. Ti3C2 nanosheets/AuPt nanoparticles (Ti3C2/AuPt NPs) with enhanced peroxidase-like activity were prepared via one-step self-reduction reaction. CBS can be hydrolyzed under acidic condition and produce –SH moieties, which could bond to Pt atoms of Ti3C2/AuPt NPs and shield the active sites of nanozyme, resulting in decreased catalytic activity. Based on the inhibitory effect on the peroxidase-like activity of Ti3C2/AuPt NPs, a colorimetric method was proposed for direct detection of CBS. Under optimal conditions, the method showed wide linear range (0.5 ng mL−1-5 μg mL−1), low limit of detection (0.342 nM), good selectivity and anti-interference ability. The feasibility of this method for practical use was confirmed by analysis of CBS in real lake water samples.

Significance

This work proposed a simple colorimetric method for selective and fast detection of CBS, which avoided employing AChE and cascade catalytic reactions, significantly lowering the detection cost and improving detection efficiency. The method showed great potential for accurate detection of CBS in actual samples, and provided a new avenue for developing nanozyme-based colorimetric method for detection of other pesticide residues.

Abstract Image

Abstract Image

基于硫丹水解行为和Ti3C2/AuPt纳米酶的选择性比色法检测
carbosulfan (CBS)是农业生产中广泛使用的氨基甲酸酯类农药,其极易分解为剧毒的呋喃,对人类健康和食品安全构成严重威胁。因此,灵敏、准确地检测CBS具有十分重要的意义。传统的色谱技术需要昂贵的仪器和复杂的样品前处理,限制了其快速检测的应用。目前的农药检测方法主要是利用乙酰胆碱酯酶(AChE)与纳米酶的级联催化反应,但选择性较差。因此,选择性地、灵敏地、快速地检测CBS仍然是一个挑战。结果利用CBS独特的水解特性和纳米酶,建立了一种选择性检测CBS的无乙酰氨基酚比色法。通过一步自还原法制备了Ti3C2纳米片/AuPt纳米颗粒(Ti3C2/AuPt NPs)。CBS可在酸性条件下水解生成-SH基团,这些-SH基团可与Ti3C2/AuPt NPs的Pt原子结合,屏蔽纳米酶的活性位点,导致催化活性降低。基于对Ti3C2/AuPt NPs过氧化物酶样活性的抑制作用,提出了一种直接检测CBS的比色法。在最佳条件下,该方法线性范围宽(0.5 ng mL−1 ~ 5 μ mL−1),检出限低(0.342 nM),具有良好的选择性和抗干扰能力。通过对真实湖泊水样中CBS的分析,证实了该方法在实际应用中的可行性。本工作提出了一种简单的选择性快速检测CBS的比色法,避免了使用AChE和级联催化反应,显著降低了检测成本,提高了检测效率。该方法显示了在实际样品中准确检测CBS的潜力,并为开发基于纳米酶的比色法检测其他农药残留提供了新的途径。
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来源期刊
Analytica Chimica Acta
Analytica Chimica Acta 化学-分析化学
CiteScore
10.40
自引率
6.50%
发文量
1081
审稿时长
38 days
期刊介绍: Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.
文献相关原料
公司名称
产品信息
麦克林
p-benzoquinone
麦克林
p-benzoquinone (PBQ)
麦克林
p-benzoquinone (PBQ)
乐研
Glyphosate (Gly)
乐研
Glyphosate
阿拉丁
Carbosulfan (CBS)
阿拉丁
Fenobucarb (FNB)
阿拉丁
Carbaryl (Car)
阿拉丁
Parathion-methyl (Para)
阿拉丁
Atrazine (Atra)
阿拉丁
Terephthalic acid (TA)
阿拉丁
Cytochrome C (Cyt C)
阿拉丁
(2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)
阿拉丁
3,3′,5,5′-tetramethylbenzidine (TMB)
阿拉丁
o-phenylenediamine (OPD)
阿拉丁
Chloroplatinic acid hexahydrate (H2PtCl6?6H2O)
阿拉丁
Gold chloride trihydrate (HAuCl4?3H2O)
阿拉丁
Carbosulfan (CBS)
阿拉丁
Fenobucarb (FNB)
阿拉丁
Carbaryl (Car)
阿拉丁
Parathion-methyl (Para)
阿拉丁
Atrazine (Atra)
阿拉丁
Terephthalic acid (TA)
阿拉丁
Cytochrome C (Cyt C)
阿拉丁
(2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)
阿拉丁
3,3′,5,5′-tetramethylbenzidine (TMB)
阿拉丁
o-phenylenediamine (OPD)
阿拉丁
Chloroplatinic acid hexahydrate (H2PtCl6?6H2O)
阿拉丁
Gold chloride trihydrate (HAuCl4?3H2O)
阿拉丁
Carbosulfan
阿拉丁
Fenobucarb
阿拉丁
Carbaryl
阿拉丁
Parathion-methyl
阿拉丁
Atrazine
阿拉丁
Terephthalic acid
阿拉丁
Cytochrome C
阿拉丁
2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid
阿拉丁
3,3′,5,5′-tetramethylbenzidine
阿拉丁
o-phenylenediamine
阿拉丁
Chloroplatinic acid hexahydrate
阿拉丁
Gold chloride trihydrate
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