Le Chen, Dongyu Zhang, Fan Yang, Xizhi Shi, Xiaohua Jiang, Tingting Hao, Qingqing Zhang, Yufang Hu, Sui Wang, Zhiyong Guo
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引用次数: 0
Abstract
Background
Foodborne pathogens, particularly Vibrio parahaemolyticus (VP) found in seafood, pose significant health risks, including abdominal pain, nausea, and even death. Rapid, accurate, and sensitive detection of these pathogens is crucial for food safety and public health. However, existing detection methods often require complex sample pretreatment, which limits their practical application. This study aims to overcome these limitations by developing a label-free magnetic relaxation switch (MRS) biosensor for the detection of VP, utilizing a photocleavable sol-gel phase transition system for improved efficiency and accuracy.
Results
In this work, a tag-free magnetic relaxation switch (MRS) biosensor was designed for the detection of Vibrio parahaemolyticus (VP), based on a photocleavable sol-gel phase transition system. A large amount of lithium acyl hypophosphite (LAP), gold nanoparticles (AuNPs), and single-stranded DNA (ssDNA) loaded on the surface of Ti3C2Tx MXene acted as the signal unit LAP-MXene@AuNPs-ssDNA. The pipette tip served as a reaction vessel, and when VP was present, Apt specifically captured VP and released the signal units. The released signal units were then injected into the low-field nuclear magnetic resonance (LF-NMR) test solution, a gel formed by crosslinking of disulfide bonds. The gel was cleaved by LAPs on the signal units under ultraviolet (UV) irradiation, triggering a gel-sol phase transition, which increased transverse relaxation time (T2), thus enabling the detection of VP. Under the optimal experimental conditions, the linear range and detection limit for VP were 102 ∼ 108 CFU/mL and 10 CFU/mL, respectively.
Significance and Novelty
The simplified biometric identification process in the pipette tip reduces errors from multiple sample transfers, enhancing efficiency. The use of photocleavable hydrogel for signal output eliminates issues associated with magnetic material aggregation, significantly improving detection precision. The assay is of good selectivity, stability reproducibility, and convenience, having a broad application prospect in the rapid detection of pathogenic bacteria in the field.
期刊介绍:
Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review.
Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.