A Photo-triggered Dual-gas Donor of Nitric Oxide and Hydrogen Sulfide with Fluorescence to Real-time Monitor Its Release

Abstract

Muti-gaseous signal molecules are significant factors to regulate various physiological and pathological processes. Therefore, studying the synergistic effects of multi-gas donors on biological systems is essential. However, different types of gas donors vary significantly in terms of gas release, including location, dose, and flux. These variations can have a dramatic impact on the biological effects of the gases and require further analysis for confirmation. Thus, a controllable dual-gas donor that enables self-reporting would be valuable for studying the biological effects of two species of gaseous signal molecules. Herin, we present a novel photo-triggered donor NHD545, which release nitric oxide and hydrogen sulfide simultaneously. Furthermore, the dual-gas release from this donor is accompanied with turn-on fluorescence, which could be utilized to monitor the NO and H2S-releasing by microscopy. With NHD545, the synerstical effect of dual-gas bioavailability is conveniently to investigate in vitro.