Intelligent DNA Nanosystem for Broad-Spectrum Oncological Typing and Therapy

IF 8.2 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Lina Zhu, Yuxi Zhang, Jiani Ding, Wenjuan Xin, Gao-Chao Fan, Zhi-Ling Song, Peisen Zhang, Xiliang Luo
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引用次数: 0

Abstract

The occurrence of multiple primary cancers in individual patients underscores the need for diagnostic and therapeutic techniques with augmented cancer-targeting selectivity and broad-spectrum antitumor effects. To address this, we develop a quadruple-input-triggered OR-AND-AND logic gated oncological nanosystem (OAA). This system employs four cancer-related markers (EpCAM, MUC1, APE1, and miR-21) to generate three distinct fluorescence signals, enabling precise differentiation of various cancer cell lines (MCF-7, HepG2, and HeLa) from normal cells (MCF-10A). Additionally, the OAA system integrates photodynamic therapy (PDT) and gene silencing strategies, allowing selective activation of Ce6 release, miR-21 gene silencing, and VEGFR2 mRNA gene silencing through the OR-AND-AND logic gating mechanism in a cancer-specific manner. This synergetic therapeutic approach induces significant apoptosis in multiple cancer cell lines while sparing normal cells, demonstrating improved cancer-targeting specificity and broad-spectrum versatility. This intelligent platform precisely types and treats diverse cancer cells, powering the future exploration of advanced diagnostic and therapeutic strategies to combat highly heterogeneous diseases.

Abstract Image

用于广谱肿瘤分型和治疗的智能DNA纳米系统
个体患者多发原发癌症的发生强调了对具有增强癌症靶向选择性和广谱抗肿瘤作用的诊断和治疗技术的需求。为了解决这个问题,我们开发了一种四输入触发的OR-AND-AND逻辑门控肿瘤纳米系统(OAA)。该系统采用四种癌症相关标志物(EpCAM、MUC1、APE1和miR-21)产生三种不同的荧光信号,使各种癌细胞系(MCF-7、HepG2和HeLa)与正常细胞(MCF-10A)精确分化。此外,OAA系统集成了光动力疗法(PDT)和基因沉默策略,通过OR-AND-AND逻辑门控机制以癌症特异性方式选择性激活Ce6释放、miR-21基因沉默和VEGFR2 mRNA基因沉默。这种协同治疗方法在多种癌细胞系中诱导了显著的细胞凋亡,同时保留了正常细胞,显示出更好的癌症靶向特异性和广谱多功能性。这个智能平台精确地分类和治疗不同的癌细胞,为未来探索先进的诊断和治疗策略提供动力,以对抗高度异质性的疾病。
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来源期刊
ACS Sensors
ACS Sensors Chemical Engineering-Bioengineering
CiteScore
14.50
自引率
3.40%
发文量
372
期刊介绍: ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.
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