Viral involvement in microbial anaerobic methane oxidation-mediated arsenic mobilization in paddy soil

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2024-12-03 DOI:10.1016/j.jhazmat.2024.136758

Youjing Wang , Di Tong , Haodan Yu , Yujie Zhou , Caixian Tang , Randy A. Dahlgren , Jianming Xu

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Abstract

Anaerobic oxidation of methane (AOM) facilitates arsenic (As) mobilization, posing a significant environmental risk. Soil viruses potentially participate in the microbial AOM process, yet their roles in methane-mediated As mobilization of paddy soil remain elusive. Here, an anaerobic microcosm study was conducted by inoculating microbial suspension with extracellular free virus and mitomycin C (MC)-induced virus, along with ¹³CH₄ injection. The results showed that extracellular free virus enhanced while MC-induced virus suppressed ¹³CH₄-mediated As mobilization. During the AOM process, both viruses inhibited ¹³CH₄ oxidation to ¹³CO₂. However, the extracellular free virus suppressed whereas the MC-induced virus enhanced ¹³CH₄ consumption, likely attributed to the viral influence on the ANME-2d abundance. The methane consumption differences were inferred to influence As reduction, as evidenced by a strong correlation between As(III) and ¹³CH₄ consumption concentrations. Moreover, virus-mediated methane assimilation into microbial biomass carbon influenced the overall microbial population. An increased abundance of Geobacter in the extracellular free virus treatment elevated net As(III) concentrations (up to 260 %) relative to treatment without virus in the presence of ¹³CH₄. In contrast, MC-induced virus led to a net 122 % reduction in As(III) concentration due to decreased Geobacter abundance. These findings provide new insights into soil viruses in microbial AOM-driven As mobilization, highlighting their crucial functions in soil ecosystems.

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病毒参与水稻土微生物厌氧甲烷氧化介导的砷动员

甲烷的厌氧氧化（AOM）促进As的动员，造成重大的环境风险。土壤病毒可能参与微生物AOM过程，但它们在甲烷介导的水稻土As动员中的作用尚不清楚。本研究通过接种细胞外游离病毒和丝裂霉素C （MC）诱导病毒的微生物悬浮液，并注射13CH4进行厌氧微生物研究。结果表明，细胞外游离病毒增强，mc诱导的病毒抑制13ch4介导的As动员。在AOM过程中，两种病毒都抑制13CH4氧化为13CO2。然而，细胞外游离病毒抑制了13CH4的消耗，而mc诱导的病毒则增强了13CH4的消耗，这可能是由于病毒对ANME-2d丰度的影响。从As（III）和13CH4消耗浓度之间的强相关性可以推断，甲烷消耗的差异会影响As的还原。此外，病毒介导的甲烷同化转化为微生物生物量碳影响了总体微生物种群。在细胞外游离病毒处理中，相对于没有13CH4存在的病毒处理，Geobacter丰度的增加使净As（III）浓度升高（高达260%）。相比之下，mc诱导的病毒由于地杆菌丰度降低，导致As（III）浓度净降低122%。这些发现为土壤病毒在微生物aom驱动的As动员中提供了新的见解，突出了它们在土壤生态系统中的重要功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.