Optimization of subcellular boron distribution measurement using UV-C imprint and neutron autoradiography in boron neutron capture therapy

IF 1.6 3区物理与天体物理 Q2 NUCLEAR SCIENCE & TECHNOLOGY

Radiation Measurements Pub Date : 2024-11-30 DOI:10.1016/j.radmeas.2024.107351

Yan Wu , Diyun Shu , Changran Geng , Ian Postuma , Xiaobin Tang , Yuan-Hao Liu

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引用次数: 0

Abstract

The subcellular distribution of boron drugs is crucial for studying radiobiological effects and microdosimetry in boron neutron capture therapy (BNCT). Accurately measuring this distribution remains a key objective. Building on the neutron autoradiography method combined with UV-C sensitization, this study aims to further optimize the approach and implement it at the BNCT center of Xiamen Humanity Hospital, with the expectation of applying it to future boron drug development. A dedicated irradiation device for neutron autoradiography was developed based on a clinical epithermal neutron beam. Optimal conditions for etching and UV-C cell imprints were investigated. After U251 cells were incubated with L-4-boronophenylalanin (BPA), cell imprints and track images were obtained under optimal conditions, and track distributions within cell structure were evaluated. The optimal etching condition involved using Potassium-Ethanol-Water (PEW) solution for 10 min, yielding track diameters of approximately 1 μm. After the poly allyl diglycol carbonate (PADC) with cultured cells was exposed to UV-C for 12 h, a clear cellular structure was imprinted on the PADC. The coupled track and cell structure images suggest that BPA may concentrate more around the U251 cell nucleus. The results demonstrate that the improved method can clearly distinguish tracks within the nucleus and cytoplasm in two-dimensional projections, enabling a more accurate evaluation of boron distribution at the subcellular scale.

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利用UV-C压印和中子放射自显影技术优化硼中子俘获治疗中的亚细胞硼分布测量

硼药物的亚细胞分布对硼中子俘获治疗（BNCT）的放射生物学效应和微剂量学研究至关重要。准确测量这种分布仍然是一个关键目标。本研究拟在中子放射自显影法结合UV-C增敏的基础上，进一步优化该方法并在厦门市人文医院BNCT中心实施，期望将其应用于未来硼类药物的开发。研制了一种基于临床超热中子束的中子自显影专用照射装置。研究了蚀刻和UV-C细胞印迹的最佳工艺条件。将U251细胞与l -4-硼苯丙氨酸（BPA）孵育后，在最佳条件下获得细胞印迹和轨迹图像，并评估细胞结构内的轨迹分布。最佳蚀刻条件是使用钾-乙醇-水（PEW）溶液10分钟，得到的径迹直径约为1 μm。将培养的聚烯丙基碳酸二甘醇（PADC）细胞暴露在UV-C下12小时后，在PADC上印迹出清晰的细胞结构。偶联轨迹和细胞结构图像表明BPA可能更多地集中在U251细胞核周围。结果表明，改进后的方法可以在二维投影中清晰地区分核内和细胞质内的轨迹，从而在亚细胞尺度上更准确地评估硼的分布。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Radiation Measurements 工程技术-核科学技术

CiteScore

4.10

自引率

20.00%

发文量

116

审稿时长

48 days

期刊介绍： The journal seeks to publish papers that present advances in the following areas: spontaneous and stimulated luminescence (including scintillating materials, thermoluminescence, and optically stimulated luminescence); electron spin resonance of natural and synthetic materials; the physics, design and performance of radiation measurements (including computational modelling such as electronic transport simulations); the novel basic aspects of radiation measurement in medical physics. Studies of energy-transfer phenomena, track physics and microdosimetry are also of interest to the journal. Applications relevant to the journal, particularly where they present novel detection techniques, novel analytical approaches or novel materials, include: personal dosimetry (including dosimetric quantities, active/electronic and passive monitoring techniques for photon, neutron and charged-particle exposures); environmental dosimetry (including methodological advances and predictive models related to radon, but generally excluding local survey results of radon where the main aim is to establish the radiation risk to populations); cosmic and high-energy radiation measurements (including dosimetry, space radiation effects, and single event upsets); dosimetry-based archaeological and Quaternary dating; dosimetry-based approaches to thermochronometry; accident and retrospective dosimetry (including activation detectors), and dosimetry and measurements related to medical applications.