Optimization of the conditions for immobilization of crude pectinase on chitin using ultrasound and glutaraldehyde and its application in grape juice clarification

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry Pub Date : 2024-11-28 DOI:10.1016/j.procbio.2024.11.032

Sohini Bera, Nipona Shill, Bhaskar Jyoti Kalita, Nandan Sit

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Abstract

The current research focuses on optimizing the immobilization of crude pectinase, produced by solid-state fermentation, onto chitin using ultrasound and glutaraldehyde as a crosslinking agent for grape juice clarification. A Box-Behnken design was used to model the effects of ultrasound time (5–15 min), ultrasound amplitude (20 – 60 %), and water-to-support ratio for ultrasound application (16−24) on immobilized crude pectinase activity and immobilization efficiency. Optimal conditions, comprising ultrasound time of 10 min, ultrasound amplitude of 20 %, and water-to-support ratio for ultrasound application of 24, yielded an immobilized crude pectinase activity of 2.19 IU/mL and 74 % immobilization efficiency. The immobilized crude pectinase showed improved kinetic properties and retained 69 % activity after three use cycles. Both free and immobilized crude pectinase enhanced juice clarity, reducing sugar, and TSS while lowering viscosity.

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超声-戊二醛固定化粗果胶酶条件的优化及其在葡萄汁澄清中的应用

目前的研究重点是利用超声波和戊二醛作为交联剂，将固态发酵产生的粗果胶酶固定化在几丁质上，以澄清葡萄汁。采用Box-Behnken设计来模拟超声时间（5-15 min）、超声振幅（20 - 60 %）和超声应用水负载比（16−24）对固定化粗果胶酶活性和固定化效率的影响。最佳条件为超声时间为10 min，超声振幅为20 %，超声水托比为24，固定化粗果胶酶活性为2.19 IU/mL，固定化效率为74 %。经3次循环后，固定化粗果胶酶的动力学性能得到改善，活性保持在69 %。游离和固定化的粗果胶酶都提高了果汁的清晰度，还原糖和TSS，同时降低了粘度。

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来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.