Cu-curcumin nanozyme for detection of Cr(vi) through an off–on strategy based on peroxidase mimicking activity †

Abstract

Hexavalent chromium ions (Cr(VI)) possess inherent toxicity and propensity for bioaccumulation in the environment. Herein, a Cu-curcumin nanozyme was synthesized via a one-pot solvothermal method. Based on the high peroxidase-like activity of the Cu-curcumin nanozyme, a novel and economical colorimetric platform has been developed for Cr(VI) assay. In the presence of hydrogen peroxide (H₂O₂), the Cu-curcumin nanozyme can catalyze the transformation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into the blue product oxTMB. 8-Hydroxyquinoline (8-HQ) can inhibit the catalysis of the Cu-curcumin/TMB/H₂O₂ system, causing the blue color to fade. With the addition of Cr(VI), the peroxidase-like activity of the Cu-curcumin nanozyme is increased significantly, leading to the restoration of the Cu-curcumin/TMB/H₂O₂ system color. After optimization of the experimental parameters, a novel colorimetric sensor for Cr(VI) assay has been successfully developed with a low limit of detection (LOD) of 31.5 nM, affirming its potential for accurate Cr(VI) quantification in environmental specimens. In addition, paper strips integrated with a smartphone platform enhance the versatility and portability of the Cr(VI) assay, achieving an LOD of 0.1 μM.