Applied research note: Optimized corticosterone extraction following feather dissolution by keratinase

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Nikolas R. Faust , Rosemary L. Walzem
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引用次数: 0

Abstract

The study aimed to develop and validate a robust, non-invasive method for assessing corticosterone (CORT) concentrations in poultry feathers as an indication of lifelong stress. Traditional sample preparation using methanol extraction showed high variability, prompting the exploration of a keratinase-based protocol. Three protocols were compared: methanol extraction (MP), original keratinase (KP), and an optimized keratinase protocol (OKP). The OKP significantly reduced intra-assay variability to 5.4 % compared to 61 % (MP) and 33 % (KP). Feather CORT levels were reliably quantified using the OKP, showing linear recovery of spiked samples (R² = 0.97). No significant correlation was found between plasma and feather CORT concentrations, underscoring the potential of feather sampling for long-term, as opposed to acute, stress assessment in poultry. This optimized method provides a valuable tool for animal welfare studies, enabling accurate and consistent measurement of feather CORT levels across different poultry strains and conditions.
应用研究笔记:优化了角化酶溶解羽毛后提取皮质酮的方法
该研究旨在开发和验证一种强大的、非侵入性的方法,用于评估家禽羽毛中的皮质酮(CORT)浓度,作为终身压力的指示。使用甲醇提取的传统样品制备显示出高可变性,促使探索基于角蛋白酶的方案。比较了三种方案:甲醇提取(MP),原始角化酶(KP)和优化角化酶方案(OKP)。与61% (MP)和33% (KP)相比,OKP显着将测定内变异性降低至5.4%。使用OKP可靠地定量羽毛CORT水平,加标样品显示线性恢复(R²= 0.97)。血浆和羽毛CORT浓度之间没有发现显著的相关性,强调了羽毛取样对家禽长期应激评估的潜力,而不是急性应激评估。这种优化的方法为动物福利研究提供了一个有价值的工具,能够准确和一致地测量不同家禽品系和条件下的羽毛CORT水平。
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来源期刊
Journal of Applied Poultry Research
Journal of Applied Poultry Research 农林科学-奶制品与动物科学
CiteScore
4.10
自引率
10.50%
发文量
80
审稿时长
104 days
期刊介绍: The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.
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