Bacillus velezensis SS-20 as a potential and efficient multifunctional agent in biocontrol, saline-alkaline tolerance, and plant-growth promotion

IF 4.8 2区 农林科学 Q1 SOIL SCIENCE
Zhaoliang Chen , Haiyun Zhang , Weiguang Lv , Siyi Zhang , Linna Du , Shuangxi Li , Hanlin Zhang , Xianqing Zheng , Juanqin Zhang , Tinglin Zhang , Naling Bai
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引用次数: 0

Abstract

The versatile strain Bacillus velezensis SS-20 was studied to determine its agricultural benefits, including its ability to antagonize fungi, promote plant growth, and tolerate saline-alkaline conditions. Firstly, strain SS-20 was found to be an antagonist of plant pathogenic fungi such as Magnaporthe oryzae, Rhizoctonia solani, and Fusarium oxysporum, with control effects up to 82.07 %, 46.57 %, and 23.09 %, respectively. The hyphae of pathogenic fungi treated with SS-20 and its fermentation product were abnormal, swollen, deformed, and distorted. Meanwhile, strain SS-20 dissolved inorganic phosphorus (phosphate solubilization efficiency, 45.95 %), and produced siderophore (siderophore units, 54.18 ± 4.19 %), and poly-γ-glutamic acid (γ-PGA) (13.35 ± 2.61 g/L). Strain SS-20 significantly increased plant height, aboveground fresh weight, and root dehydrogenase activity compared to the control treatment under non-saline conditions (p < 0.05). In addition, strain SS-20 showed significant saline-alkaline tolerance, surviving in 10 % NaCl and pH 9.5, and enhanced plant growth under simulated saline-alkali conditions. Whole-genome sequencing analysis revealed that the chromosome of B. velezensis SS-20 consists of 3,929,792-bp with 46.5 % guanine-cytosine content and 4015 coding sequences. The antiSMASH analysis identified 12 gene clusters within strain SS-20 that encode antimicrobial compounds such as surfactin, fengycin, butirosin A/butirosin B, macrolactin H, bacillibactin, difficidin, and bacilysin, thereby corroborating the strain's biocontrol capabilities. Genes related to plant growth promotion, including siderophores, γ-PGA, indole acetic acid production, phosphate solubilization, glycine-betaine production, and Na+/H+ antiporters, were also annotated. In conclusion, B. velezensis SS-20 represents a promising biotic resource for enhancing plant growth and protecting against environmental stresses.
velezensis SS-20是一种具有生物防治、耐盐碱和促进植物生长潜力的高效多功能药剂
对多功能菌株velezensis SS-20进行了研究,以确定其农业效益,包括其拮抗真菌,促进植物生长和耐受盐碱条件的能力。首先,菌株SS-20对稻瘟病菌(Magnaporthe oryzae)、番茄丝核菌(Rhizoctonia solani)和尖孢镰刀菌(Fusarium oxysporum)具有拮抗作用,防效分别为82.07%、46.57%和23.09%;经SS-20及其发酵产物处理后,病原菌菌丝出现异常、肿胀、变形、扭曲等现象。菌株SS-20溶解无机磷(磷酸盐溶解效率45.95%),产生铁载体(铁载体单位54.18±4.19%)和聚γ-谷氨酸(γ-PGA)(13.35±2.61 g/L)。菌株SS-20在无盐条件下显著提高了植株高度、地上鲜重和根系脱氢酶活性(p <;0.05)。此外,菌株SS-20表现出显著的耐盐碱性,在10% NaCl和pH 9.5条件下均能存活,并在模拟盐碱条件下促进植株生长。全基因组测序结果显示,该菌株全长3929792 bp,鸟嘌呤-胞嘧啶含量46.5%,编码序列4015条。抗smash分析在菌株SS-20中鉴定出12个基因簇,编码抗菌化合物,如surfactin、fengycin、butirosin A/butirosin B、macrolactin H、bacillibactin、difficidin和bacilysin,从而证实了菌株的生物防治能力。与植物生长促进相关的基因,包括铁载体、γ-PGA、吲哚乙酸生成、磷酸盐溶解、甘氨酸-甜菜碱生成和Na+/H+反转运蛋白,也进行了注释。综上所述,白僵菌SS-20在促进植物生长和抵御环境胁迫方面是一种很有前景的生物资源。
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来源期刊
Applied Soil Ecology
Applied Soil Ecology 农林科学-土壤科学
CiteScore
9.70
自引率
4.20%
发文量
363
审稿时长
5.3 months
期刊介绍: Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.
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