The Regulatory Effect of Insulin-Like Growth Factor-2 on Hypothalamic Gonadotropin-Releasing Hormone Neurons during the Pubertal Period.

IF 2.5 4区医学 Q3 NEUROSCIENCES

Journal of integrative neuroscience Pub Date : 2024-11-20 DOI:10.31083/j.jin2311208

Xiaoli Dai, Yuanyuan He, Xinghui Han, Wen Sun, Jian Yu, Yating Lin, Yonghong Wang

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引用次数: 0

Abstract

Background: The insulin-like growth factor (IGF) system plays a vital role in regulating gonadotropin-releasing hormone (GnRH), whether the IGF2 can act on the GnRH neurons during the pubertal period is unclear.

Methods: Central precocious puberty (CPP) rats were induced by danazol, and when the rats met the first diestrus, they were euthanized and tissues were collected. GT1-7 cells were cultured and treated with 0, 1, 10 ng/mL IGF2 for 4 hours and the changes in GnRH were measured. Mice were injected intracerebroventricularly with IGF2 (15 ng/g, 5 μL) or with the same dose of phosphate buffered saline (PBS), after eight hours, they were euthanized and tissues collected.

Results: CPP rats had increased expression of IGF2 and GnRH mRNA and their respective proteins in the preoptic area (POA) of the hypothalamus. Treatment of GT1-7 cells with 10 ng/mL of IGF2 increased GnRH mRNA and protein expression, and GnRH concentration in the culture medium. Injection of IGF2 protein into the lateral ventricle of mice increased the expression of GnRH mRNA and protein in the POA.

Conclusions: IGF2 may upregulate the synthesis of GnRH during the pubertal period, and may also take part in the pathology of CPP.

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胰岛素样生长因子-2对青春期下丘脑促性腺激素释放激素神经元的调控作用。

背景：胰岛素样生长因子（IGF）系统在调节促性腺激素释放激素（GnRH）中起着至关重要的作用，IGF2在青春期是否能作用于GnRH神经元尚不清楚。方法：采用达那唑诱导中枢性性早熟（CPP）大鼠，第一次发病后处死，收集组织。分别用0、1、10 ng/mL IGF2处理GT1-7细胞4小时，测定GnRH的变化。小鼠脑室内注射IGF2 （15 ng/g, 5 μL）或相同剂量的磷酸盐缓冲盐水（PBS）， 8小时后安乐死并收集组织。结果：CPP大鼠下丘脑视前区（POA） IGF2和GnRH mRNA及其相关蛋白表达升高。用10 ng/mL IGF2处理GT1-7细胞，GnRH mRNA和蛋白表达增加，培养基中GnRH浓度增加。向小鼠侧脑室注射IGF2蛋白可增加POA中GnRH mRNA和蛋白的表达。结论：IGF2可能上调青春期GnRH的合成，并可能参与CPP的病理过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of integrative neuroscience 医学-神经科学

CiteScore

2.80

自引率

5.60%

发文量

173

审稿时长

2 months

期刊介绍： JIN is an international peer-reviewed, open access journal. JIN publishes leading-edge research at the interface of theoretical and experimental neuroscience, focusing across hierarchical levels of brain organization to better understand how diverse functions are integrated. We encourage submissions from scientists of all specialties that relate to brain functioning.