Yifan Zeng, Shiyong Sun, Sen Lin, Rui Lv, Ke Wang, Jianjun Deng
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引用次数: 0
Abstract
Recently, the synthesis of nanozymes-enzyme microsystems with high catalytic stability provides new opportunities for treating diverse pollutants in complex aquatic systems. Herein, a carboxyl-functionalized CuMn bimetallic nanozyme-enzyme microsystem (CMAC@Lipase) was successfully constructed by combining copper‑manganese based aminoclays (CMAC) with lipase. This system exhibited laccase-like catalytic activity facilitated by CuMn electron transfer, while enhancing lipase stability via its carrier function. Under alkaline conditions at pH 10, CMAC@Lipase catalyzed the hydrolysis of p-NPP (280 nm) to produce p-NP (400 nm), and subsequently reduced p-NP to p-AP within 30 min with the assistance of NaBH4. Furthermore, it effectively degraded 72.8 % of dimethyl phthalate (DMP) at 40 mg·L-1 under alkaline conditions within 48 h, maintaining a 53.5 % degradation rate after 10 reuse cycles. This work provided a new strategy for the design of nanozyme-enzyme microsystems and a new research idea for the efficient treatment of contaminants in actual aqueous environments.
期刊介绍:
The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.