A simply synthesized, silver ions-doped porous gold microparticles-based SERS aptamer sensor for ultrasensitive and broad-range quantitative detection of IL-6

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-30 DOI:10.1016/j.aca.2024.343504

Bixia Liang, Zhixi Huang, Lingyan Cheng, Jin Quan, Zongxia Mou, Nan Li, Xin Cui

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Abstract

Background

The multifunctional cytokine interleukin-6 (IL-6) plays a pivotal role in chronic and acute inflammatory responses, underscoring the importance of accurately determining IL-6 levels for early diagnosis, prevention, and treatment of inflammation.

Results

This study developed a versatile and innovative single-particle surface-enhanced Raman spectroscopy (SERS) sensing platform for the precise and sensitive quantification of IL-6 in complex samples using a novel one-pot synthesized, silver ions-doped three-dimensional porous gold microparticles (PGMs) with abundant hot spots for robust SERS enhancement. By rationally designing rich cytosine-Ag⁺-cytosine base pairs between IL-6 aptamers and complementary chains on the PGMs, we harnessed the SERS-enhancing effect to achieve highly sensitive and specific IL-6 quantification within a wide range of 10^-9 to 10^-4 mg/mL and a limit of detection (LOD) of 0.093 pg/mL. Moreover, the accurate detection of IL-6 in spiked serum samples exhibited a remarkable recovery rate ranging from 97.2% and 107.6%.

Significance

This aptamer sensor provides an alternative tool for cost-effective, sensitive and broad-range IL-6 detection, which would contribute to the early detection of inflammation and pave the way for timely interventions and improved patient outcomes.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.