Dairy-based multi-strain probiotic community successfully mitigated obesity-related gut microbiota dysbiosis in vitro (CoMiniGut).

Muhammad Nadeem Khan, Zhuqing Xie, Syeda Momna Batool Bukhari, Dennis Sandris Nielsen, Muhammad Imran
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Abstract

Introduction. Obesity is a global health concern, affecting individuals of all ages and genders. One promising strategy to combat obesity is by addressing gut microbiota dysbiosis, with probiotics being a reliable intervention. However, single-strain probiotics may not effectively modulate the complex microbial communities in the gut, suggesting the need for multi-strain approaches.Gap Statement. Probiotics are known to benefit gut health; however, the efficacy of single-strain probiotics in modulating gut microbiota is limited. Multi-strain probiotic community (MSPC) may offer a more effective approach for addressing obesity-related gut dysbiosis, but its specific effects on individuals and microbial diversity require further investigation.Aim. This study aimed to evaluate the potential of a dairy-origin MSPC in modulating obesity-related gut microbiota from lean and obese Pakistani volunteers using a simulated CoMiniGut model.Methodology. Gut microbiota from lean and obese volunteers were treated with MSPC in a simulated CoMiniGut system. Bacterial counts, microbial diversity (α- and β-diversity) and microbial community composition were analysed pre- and post-treatment. The impact of MSPC on specific bacterial genera and microbial metabolites was assessed, with statistical significance determined (P≤0.05).Results. The effect of MSPC was individualized, reducing bacterial counts in lean 1 and lean 2 samples, while significantly increasing bacterial counts in obese 2 and obese 3 samples (P≤0.05). MSPC significantly improved α-diversity in lean 2, lean 3, obese 2 and obese 3 samples (P≤0.05). Proteobacteria decreased in the lean group and increased in the obese group post-MSPC treatment. In the lean group, pathogenic bacteria such as Klebsiella, Escherichia and Enterobacter were significantly reduced (P≤0.05), whereas beneficial bacteria like Bifidobacterium and Lactobacillus increased significantly in the obese group (P≤0.05). Among the selected metabolites, only butanoic acid was detected in all tested samples, with MSPC affecting metabolite concentrations and types.Conclusion. MSPC demonstrated a potential for modulating gut microbiota dysbiosis in both lean and obese individuals, with effects on bacterial counts, microbial diversity and metabolite concentrations. MSPC could serve as a promising option for personalized the modulation of gut microbiota in obesity management.

基于乳制品的多菌株益生菌群落在体外成功减轻了与肥胖相关的肠道微生物群失调(CoMiniGut)。
介绍。肥胖是一个全球性的健康问题,影响着所有年龄和性别的个体。对抗肥胖的一个有希望的策略是通过解决肠道微生物群失调,益生菌是一种可靠的干预。然而,单菌株益生菌可能不能有效调节肠道内复杂的微生物群落,这表明需要多菌株方法。差距的声明。众所周知,益生菌有益于肠道健康;然而,单一菌株益生菌调节肠道菌群的功效是有限的。多菌株益生菌群落(MSPC)可能为解决肥胖相关肠道生态失调提供更有效的方法,但其对个体和微生物多样性的具体影响有待进一步研究。本研究旨在通过模拟CoMiniGut模型,评估乳源性MSPC在调节瘦弱和肥胖巴基斯坦志愿者的肥胖相关肠道微生物群方面的潜力。瘦和肥胖志愿者的肠道微生物群在模拟CoMiniGut系统中接受MSPC治疗。分析处理前后细菌数量、微生物多样性(α-和β-多样性)和微生物群落组成。评价MSPC对特定菌属和微生物代谢物的影响,差异有统计学意义(P≤0.05)。MSPC的效果是个体化的,可降低瘦肉1号和瘦肉2号样品的细菌计数,显著增加肥胖2号和肥胖3号样品的细菌计数(P≤0.05)。MSPC显著提高了瘦2型、瘦3型、肥胖2型和肥胖3型样品α-多样性(P≤0.05)。mspc治疗后,瘦组的变形菌群减少,肥胖组的变形菌群增加。瘦肉组克雷伯氏菌、埃希氏菌、肠杆菌等致病菌显著减少(P≤0.05),肥胖组双歧杆菌、乳酸杆菌等有益菌显著增加(P≤0.05)。在所选择的代谢物中,所有样品中仅检测到丁酸,MSPC影响代谢物浓度和类型。MSPC证明了在瘦肉和肥胖个体中调节肠道微生物群失调的潜力,对细菌数量、微生物多样性和代谢物浓度都有影响。MSPC可以作为肥胖管理中肠道微生物群个性化调节的一个有前途的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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