The Role of Fibrinogen and Platelets in Mouse Liver Ischemia-Reperfusion Injury: Distribution and Pathophysiological Insights

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2024-12-01 DOI:10.1016/j.transproceed.2024.10.045

Meng-Qi Dong , Zhi-Hao Huang , Zhi-min Liu , Yuan Lin , Feng-Yong Liu , Wei-Jie Zhou

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引用次数: 0

Abstract

Liver ischemia-reperfusion (I/R) injury is a critical issue in clinical settings, particularly in liver transplantation and resection, leading to severe hepatocellular dysfunction and organ failure. This study investigates the role of fibrinogen and platelets in liver I/R injury, focusing on their distribution and pathophysiological impact within liver lobules. Using a mouse model, we examined the expression and localization of fibrinogen and platelets at various time points postreperfusion. In normal liver, fibrinogen is predominantly expressed in hepatic sinusoids near the portal vein, with sparse platelet distribution. Following I/R injury, fibrinogen expression significantly increases in hepatic sinusoids and hepatocytes, accompanied by substantial platelet aggregation and embolization, particularly in Zone 1 of the liver lobules. These findings highlight the zonal heterogeneity of fibrinogen distribution and its regulatory function in platelet adhesion and microthrombi formation. This study provides crucial insights for developing therapeutic strategies targeting fibrinogen and platelet interactions to mitigate liver I/R injury.

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纤维蛋白原和血小板在小鼠肝缺血再灌注损伤中的作用：分布和病理生理观察。

肝缺血再灌注（I/R）损伤是临床环境中的一个关键问题，特别是在肝移植和肝切除术中，导致严重的肝细胞功能障碍和器官衰竭。本研究探讨了纤维蛋白原和血小板在肝I/R损伤中的作用，重点研究了它们在肝小叶内的分布和病理生理影响。利用小鼠模型，我们检测了灌注后不同时间点纤维蛋白原和血小板的表达和定位。在正常肝脏中，纤维蛋白原主要表达于门静脉附近的肝窦，血小板分布稀疏。I/R损伤后，肝窦和肝细胞中的纤维蛋白原表达显著增加，并伴有大量血小板聚集和栓塞，尤其是在肝小叶的1区。这些发现突出了纤维蛋白原分布的区域异质性及其在血小板粘附和微血栓形成中的调节功能。这项研究为开发针对纤维蛋白原和血小板相互作用的治疗策略以减轻肝I/R损伤提供了重要的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.