High-speed two-photon microscopy with adaptive line-excitation.

IF 8.4 1区 物理与天体物理 Q1 OPTICS
Optica Pub Date : 2024-08-15 eCollection Date: 2024-08-20 DOI:10.1364/OPTICA.529930
Yunyang Li, Shu Guo, Ben Mattison, Junjie Hu, Kwun Nok Mimi Man, Weijian Yang
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引用次数: 0

Abstract

We present a two-photon fluorescence microscope designed for high-speed imaging of neural activity at cellular resolution. Our microscope uses an adaptive sampling scheme with line illumination. Instead of building images pixel by pixel via scanning a diffraction-limited spot across the sample, our scheme only illuminates the regions of interest (i.e., neuronal cell bodies) and samples a large area of them in a single measurement. Such a scheme significantly increases the imaging speed and reduces the overall laser power on the brain tissue. Using this approach, we performed high-speed imaging of the neuronal activity in mouse cortex in vivo. Our method provides a sampling strategy in laser-scanning two-photon microscopy and will be powerful for high-throughput imaging of neural activity.

自适应线激发的高速双光子显微镜。
我们提出了一种双光子荧光显微镜,用于细胞分辨率下神经活动的高速成像。我们的显微镜采用线照明的自适应采样方案。我们的方案不是通过扫描样品上的衍射极限点逐像素地构建图像,而是只照亮感兴趣的区域(即神经元细胞体),并在一次测量中对其中的大片区域进行采样。这种方案显著提高了成像速度,并降低了对脑组织的总体激光功率。利用这种方法,我们在体内对小鼠皮层的神经元活动进行了高速成像。我们的方法为激光扫描双光子显微镜提供了一种采样策略,将为神经活动的高通量成像提供强大的支持。
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来源期刊
Optica
Optica OPTICS-
CiteScore
19.70
自引率
2.90%
发文量
191
审稿时长
2 months
期刊介绍: Optica is an open access, online-only journal published monthly by Optica Publishing Group. It is dedicated to the rapid dissemination of high-impact peer-reviewed research in the field of optics and photonics. The journal provides a forum for theoretical or experimental, fundamental or applied research to be swiftly accessed by the international community. Optica is abstracted and indexed in Chemical Abstracts Service, Current Contents/Physical, Chemical & Earth Sciences, and Science Citation Index Expanded.
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