Detection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2025-02-01 Epub Date: 2024-11-29 DOI:10.1080/17460913.2024.2432179

Aishwarya Soni, Kiran Nehra, Bhawna Dahiya, Anam Rais, Tulika Prasad, Anjum Gahlaut, Vikas Raj, Reetu Sheoran, Aparna Parmar, Promod K Mehta

{"title":"Detection of MPT-64 protein in pleural tuberculosis cases by magnetic bead-gold nanoparticle-PCR amplified immunoassay.","authors":"Aishwarya Soni, Kiran Nehra, Bhawna Dahiya, Anam Rais, Tulika Prasad, Anjum Gahlaut, Vikas Raj, Reetu Sheoran, Aparna Parmar, Promod K Mehta","doi":"10.1080/17460913.2024.2432179","DOIUrl":null,"url":null,"abstract":"Aim: Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.Methods: We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the Mycobacterium tuberculosis MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy.Results: We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (p < 0.01) than magneto-ELISA and GeneXpert.Conclusion: This is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"107-115"},"PeriodicalIF":2.5000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792811/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/17460913.2024.2432179","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/29 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: Diagnosis of pleural tuberculosis (TB) is challenging; thus, an efficient method is urgently needed.

Methods: We developed a magnetic-bead-gold nanoparticle-PCR amplified immunoassay (MB-AuNP-I-PCR, liquid system) to detect the Mycobacterium tuberculosis MPT-64 protein in pleural TB patients. AuNPs functionalized with detection antibodies/oligonucleotides were characterized by UV-vis spectroscopy, Transmission/Scanning electron microscopy, Fourier-transform infrared spectrometer, ELISA, and PCR, whereas MBs conjugated with detection antibodies were validated by magneto-ELISA/UV-vis spectroscopy.

Results: We utilized the MB-AuNP-I-PCR for MPT-64 detection in 99 clinical specimens which displayed 85.2% sensitivity and 97.8% specificity to diagnose pleural TB cases. Markedly, the sensitivity achieved by MB-AuNP-I-PCR was noticeably higher (p < 0.01) than magneto-ELISA and GeneXpert.

Conclusion: This is a preliminary report to diagnose pleural TB cases by MB-AuNP-I-PCR with promising results that require further corroboration in a higher number of specimens.

查看原文本刊更多论文

磁珠-金纳米颗粒- pcr扩增免疫分析法检测胸膜结核患者MPT-64蛋白。

目的：胸膜结核（TB）的诊断具有挑战性；因此，迫切需要一种有效的方法。方法：建立磁珠-金纳米颗粒- pcr扩增免疫分析法（MB-AuNP-I-PCR，液体系统）检测胸膜结核患者结核分枝杆菌MPT-64蛋白。检测抗体/寡核苷酸功能化的AuNPs通过紫外可见光谱、透射/扫描电镜、傅里叶变换红外光谱仪、ELISA和PCR进行了表征，而与检测抗体偶联的MBs通过磁ELISA/紫外可见光谱进行了验证。结果：应用MB-AuNP-I-PCR对99例临床标本进行MPT-64检测，诊断胸膜结核的敏感性为85.2%，特异性为97.8%。结论：这是一篇用MB-AuNP-I-PCR诊断胸膜结核病例的初步报告，结果令人满意，但需要更多标本的进一步证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.