Pseudomonas chlororaphis ZH2: Evaluation of the Biocontrol Potential of Continuous Cropping Obstacles on the Basis of Genome Analysis, Autotoxic Substance Degradation and In Vitro Antifungal Activity.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Rong Xiao, Chunfen Zhang, Shu Deng, Ciqiong Niu, Quan Li, Junjie Sun, Juantao Liu, Jianping Wang
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Abstract

The accumulation of autotoxic substances and fungal pathogens in soil are the two leading causes of continuous cropping obstacles. In this context, the use of beneficial strains for the biological control of continuous cropping obstacles is a promising research direction. In this work, the functions of Pseudomonas chlororaphis ZH2 in antagonizing pathogenic fungi and degrading autotoxic substances during continuous cropping were studied via genome-wide sequence analysis, antifungal activity in vitro, and autotoxic substances degrading tests. The results revealed that the genome of ZH2 contains 7,249,755 base pairs, has a GC content of 62.61%, and is predicted to contain 6551 genes. The phylogenetic results of 16S rRNA, atpD, recA, and carA gene analysis and the average nucleotide identity analysis revealed that ZH2 belongs to P. chlororaphis subsp. aurantiaca. KEGG analysis revealed that some genes of ZH2 were annotated to the biodegradation pathway of benzoate, p-hydroxybenzoic acid, and other autotoxic substances. Nineteen types of secondary metabolite biosynthesis-related gene clusters were predicted to exist in ZH2 via antiSMASH software, and it is predicted that ZH2 can produce a variety of secondary metabolites with antifungal and siderophore functions. The autotoxic substance degradation tests confirmed that ZH2 can degrade benzoate, p-hydroxybenzoic acid, vanillin, and vanillic acid, and the antagonism tests confirmed that ZH2 can inhibit the growth of pathogenic fungi such as Fusarium solani, Rhizopus oryzea, Pestalotiopsis mangiferae, Fusarium oxysporum f.sp. cucumerinum and Botryosphaeria dothidea. These results show that ZH2 degrades autotoxic substances and antagonizes pathogens. Therefore, it is a promising strain that can be used for the biological control of continuous cropping obstacles.

绿绿假单胞菌ZH2:基于基因组分析、自毒物质降解和体外抗真菌活性评价连作障碍生物防治潜力
土壤中自毒物质和真菌病原菌的积累是造成连作障碍的两个主要原因。在此背景下,利用有益菌种生物防治连作障碍是一个很有前景的研究方向。本文通过全基因组序列分析、体外抗真菌活性和自毒物质降解试验,研究了绿假单胞菌ZH2在连作中拮抗病原菌和降解自毒物质的功能。结果显示,ZH2基因组包含7249755个碱基对,GC含量为62.61%,预计包含6551个基因。16S rRNA、atpD、recA和carA基因分析及平均核苷酸同源性分析表明,ZH2属于绿皮拟虫亚种。树蛙。KEGG分析显示,ZH2的部分基因与苯甲酸酯、对羟基苯甲酸等自毒物质的生物降解途径有关。通过antiSMASH软件预测ZH2中存在19种次生代谢物生物合成相关基因簇,预测ZH2可产生多种具有抗真菌和铁载体功能的次生代谢物。自毒物质降解试验证实,ZH2可降解苯甲酸酯、对羟基苯甲酸、香兰素和香草酸,拮抗试验证实,ZH2可抑制番茄枯萎菌、米根霉、芒果拟多毛孢、尖孢镰刀菌等病原真菌的生长。黄瓜和葡萄球孢菌。这些结果表明,ZH2能降解自毒物质并拮抗病原体。因此,它是一种很有希望用于连作障碍生物防治的菌株。
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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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