Comparison of rose bengal-green light scleral crosslinking in rabbit eyes using different infiltration protocols - An Ex Vivo study

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2024-11-26 DOI:10.1016/j.exer.2024.110183

Rongrong Gao , Yuyan Huang , Aodong Chen , Qingqing Jiang , Shengnan Ding , Keith M. Meek , Qinmei Wang , A-Yong Yu , Jinhai Huang

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Abstract

Different concentrations and infiltration times of rose bengal (Rb) were assessed for their impact on penetration depth and crosslinking efficacy in rabbit sclera. Fresh rabbit eyes were used. Rb solution with concentrations of 0.1%–0.9% were applied for 5–30 min to infiltrate the sclera. The penetration depth of Rb was observed with confocal microscopy. After infiltration, the sclera was irradiated by green light for crosslinking. The sclera's biomechanical stiffness and the resistance to enzyme digestion post-treatment were evaluated. Histopathological analysis and transmission electron microscopy were performed to observe the morphology. As the infiltration time increased, the penetration depth and the fluorescence intensity of the Rb in sclera increased. After 32 h, 48.6% of the scleral tissue was undissolved in the 0.5% Rb-10min group, followed by the 0.1% Rb-20min group (13.8%) and 0.05% Rb-30min group (7.7%). At 8% strain, the Young's modulus of the 0.05%Rb-30min, the 0.1% Rb-20min and the 0.5% Rb-10min group were respectively 1.77, 2.45 and 3.19 times greater than that of the untreated group. There were no morphological differences between the experimental group and the untreated group. RG-SXL significantly increased the diameter of large collagen fibers in the middle and inner layers of the sclera. Ultimately, 0.5% Rb infiltration for 10 min achieves an appropriate infiltration depth and crosslinking effect, and may thus be a feasible schedule for scleral crosslinking.

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不同浸润方案下兔眼玫瑰红-绿光巩膜交联的体外研究。

研究了不同浓度和浸渍时间对红曲（Rb）在家兔巩膜中的渗透深度和交联效果的影响。使用新鲜的兔眼。应用浓度为0.1% ~ 0.9%的Rb溶液浸润巩膜5 ~ 30min。用共聚焦显微镜观察Rb的穿透深度。浸润后，用绿光照射巩膜进行交联。观察术后巩膜的生物力学刚度和酶消化阻力。采用组织病理学和透射电镜观察组织学形态。随着浸润时间的增加，Rb在巩膜内的穿透深度和荧光强度增加。32 h后，0.5% Rb-10min组巩膜组织未溶解率为48.6%，其次是0.1%Rb-20min组（13.8%）和0.05%Rb-30min组（7.7%）。在8%应变下，0.05%Rb-30min、0.1%Rb-20min和0.5%Rb-10min组的杨氏模量分别是未处理组的1.77、2.45和3.19倍。实验组与未治疗组之间无形态学差异。RG-SXL显著增加了巩膜中层和内层大胶原纤维的直径。最终，0.5% Rb浸润10min可获得合适的浸润深度和交联效果，可能是可行的巩膜交联时间。

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.