Combined catalytic strategies applied to in-office tooth bleaching: whitening efficacy, cytotoxicity, and gene expression of human dental pulp cells in a 3D culture model.

IF 3.1 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Rafael Antonio de Oliveira Ribeiro, Victória Peruchi, Igor Paulino Mendes Sores, Filipe Koon Wu Mon, Diana Gabriela Soares, Josimeri Hebling, Carlos Alberto de Souza Costa
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引用次数: 0

Abstract

Objectives: To evaluate the feasibility of using a 3D model with human dental pulp cells (HDPCs) to compare bleaching therapies and assess whether coating enamel with a nanofiber scaffold (NS) and polymeric catalyst primer (PCP), combined with violet LED (LEDv) irradiation, enhances bleaching efficacy (BE) and reduces cytotoxicity (CT).

Materials and methods: After using NS + PCP to cover enamel of enamel/dentin discs adapted to artificial pulp chambers containing 3D culture with HDPCs, a bleaching gel with 35%H2O2 was applied and then irradiated with LEDv. The following groups were established (n = 8): NC - no treatment; PC- 35%H2O2 for 45 min, and EXP: NS + PCP + 35%H2O2 + LEDv for 15 min. The study evaluated BE (ΔE00 and ΔWID), CT (alamarBlue), and HDPCs gene modulation (TNF, IL1B, PTGS2, IL8, IL6, PPRAG, HMOX1, DSPP, DMP1, SPP1, BGLAP, and ALPL; RTqPCR).

Results: BE showed no significant difference between PC and EXP (p > 0.05). EXP had lower oxidative stress, higher cell viability, reduced inflammatory marker expression, and increased mineralization marker expression compared to PC (p < 0.05).

Conclusion: The 3D model using HDPCs effectively compared bleaching protocols. Coating enamel with NS + PCP and applying violet LED (LEDv) reduced bleaching time from 45 to 15 min while lowering cytotoxicity compared to conventional in-office treatments.

Clinical relevance: This study shows that a 3D model with human dental pulp cells (HDPCs) effectively compares tooth bleaching protocols. The combination of a nanofiber scaffold with violet LED (LEDv) reduces bleaching time, as well as minimizes cytotoxicity and inflammation, offering a safer alternative to conventional treatments.

应用于办公室牙齿漂白的联合催化策略:三维培养模型中人牙髓细胞的美白功效、细胞毒性和基因表达
目的:评价利用人牙髓细胞(HDPCs)三维模型比较漂白方法的可行性,并评估纳米纤维支架(NS)和聚合触媒底漆(PCP)结合紫光LED (LEDv)照射涂层牙釉质是否能提高漂白效果(BE)和降低细胞毒性(CT)。材料与方法:采用NS + PCP覆盖HDPCs三维培养人工牙髓腔的牙釉质/牙本质盘牙釉质后,涂上含35%H2O2的漂白凝胶,再用LEDv照射。设各组(n = 8): NC -不处理;PC- 35%H2O2 45分钟,EXP: NS + PCP + 35%H2O2 + LEDv 15分钟。研究评估BE (ΔE00和ΔWID)、CT (alamarBlue)和HDPCs基因调节(TNF、IL1B、PTGS2、IL8、IL6、PPRAG、HMOX1、DSPP、DMP1、SPP1、BGLAP和ALPL;RTqPCR)。结果:PC与EXP的BE值无显著差异(p < 0.05)。与PC相比,EXP具有较低的氧化应激、较高的细胞活力、较低的炎症标志物表达和较高的矿化标志物表达(p)。用NS + PCP涂层牙釉质并应用紫色LED (LEDv)将漂白时间从45分钟减少到15分钟,同时与传统的办公室处理相比降低了细胞毒性。临床意义:本研究表明,具有人类牙髓细胞(HDPCs)的3D模型有效地比较了牙齿漂白方案。纳米纤维支架与紫色LED (LEDv)的结合减少了漂白时间,并最大限度地减少了细胞毒性和炎症,为传统治疗提供了更安全的替代方案。
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来源期刊
Clinical Oral Investigations
Clinical Oral Investigations 医学-牙科与口腔外科
CiteScore
6.30
自引率
5.90%
发文量
484
审稿时长
3 months
期刊介绍: The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.
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