Bone marrow mesenchymal stem cells-derived exosomes promote spinal cord injury repair through the miR-497-5p/TXNIP/NLRP3 axis

IF 2.9 4区生物学 Q3 CELL BIOLOGY

Journal of Molecular Histology Pub Date : 2024-11-29 DOI:10.1007/s10735-024-10289-z

JiXu Xu, Jun Zhang, QiaoYun Liu, Bin Wang

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引用次数: 0

Abstract

Bone marrow mesenchymal stem cells (BMSCs) indicate a repairing prospect to treat spinal cord injury, a major traumatic disease. This study investigated the repair effect of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) on spinal cord injury. BMSCs were collected to extract BMSC-Exos which were identified by different means. The SCI model of rats was established, the motor behavior was scored by BBB field test, and the spinal cord tissues were separated and stained by HE, Nissl, and Tunel, respectively, as well as analyzed to measure inflammatory and oxidative stress responses. PC12 cells were co-cultured with Exos and analyzed by CCK-8 and flow cytometry to measure cell proliferation and apoptosis. BMSC-Exos improved SCI in rats with the recovery of motor function, alleviation of pathological conditions, and reduction of apoptosis, inflammatory responses, and oxidative stress. BMSC-Exos increased miR-497-5p expression, and miR-497-5p overexpression strengthened the protective effect of BMSC-Exos on SCI. miR-497-5p targeted inactivation of TXNIP/NLRP3 pathway. TXNIP saved the repair effect of miR-497-5p-carrying BMSC-Exos on SCI rats. miR-497-5p-carrying BMSC-Exos alleviated apoptosis and induced proliferation of H₂O₂-treated PC12 cells. BMSC-Exos promote SCI repair via the miR-497-5p/TXNIP/NLRP3 axis, which may be a target for alleviating SCI-associated nerve damage.

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骨髓间充质干细胞来源的外泌体通过miR-497-5p/TXNIP/NLRP3轴促进脊髓损伤修复。

骨髓间充质干细胞（BMSCs）在治疗脊髓损伤这一重大创伤性疾病中具有广阔的应用前景。本研究探讨骨髓间充质干细胞源性外泌体（BMSC-Exos）对脊髓损伤的修复作用。收集骨髓间充质干细胞，提取骨髓间充质干细胞- exos，用不同的方法鉴定。建立大鼠脊髓损伤模型，采用BBB场试验对运动行为进行评分，分别对脊髓组织进行HE、Nissl、Tunel染色，并分析测量炎症和氧化应激反应。PC12细胞与Exos共培养，CCK-8和流式细胞术检测细胞增殖和凋亡情况。BMSC-Exos通过恢复大鼠的运动功能、减轻病理状况、减少细胞凋亡、炎症反应和氧化应激来改善脊髓损伤。BMSC-Exos增加了miR-497-5p的表达，miR-497-5p过表达增强了BMSC-Exos对SCI的保护作用。miR-497-5p靶向TXNIP/NLRP3通路失活。TXNIP挽救了携带mir -497-5p的BMSC-Exos对SCI大鼠的修复作用。携带mir -497-5p的BMSC-Exos可减轻h2o2处理的PC12细胞的凋亡并诱导增殖。BMSC-Exos通过miR-497-5p/TXNIP/NLRP3轴促进SCI修复，这可能是减轻SCI相关神经损伤的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.