Genetic Diversity of Oreochromis shiranus (Boulenger 1905) After Six Consecutive Generations of Selective Breeding Compared With Corresponding Wild Populations

IF 1.9 4区农林科学 Q2 FISHERIES

Aquaculture Research Pub Date : 2024-11-28 DOI:10.1155/are/4440384

Thomson Sanudi, Clint Rhode, Rouvay Roodt-Wilding, Alfred Maluwa

{"title":"Genetic Diversity of Oreochromis shiranus (Boulenger 1905) After Six Consecutive Generations of Selective Breeding Compared With Corresponding Wild Populations","authors":"Thomson Sanudi, Clint Rhode, Rouvay Roodt-Wilding, Alfred Maluwa","doi":"10.1155/are/4440384","DOIUrl":null,"url":null,"abstract":"<div>\n <p>Aquaculture in sub-Saharan African accounts for less than 2% of world aquaculture production. The use of unimproved species limits the growth of aquaculture. In order to increase production, there is a need to develop aquaculture species that show vigor and adapt to different culture conditions. A genetic improvement program for <i>Oreochromis shiranus</i> was initiated at the National Aquaculture Center (NAC), in Zomba, Malawi. The program used pedigree records during mate allocation to avoid mating of siblings. After six consecutive generations of selection, a sample of 32 fish was taken for genetic analysis using a panel of 16 microsatellite markers. Genetic diversity and population differentiation indices of the sixth filial generation (F6) were compared with wild fish populations sampled from the corresponding locations from which the initial brood stock was collected. The study results show that the genetic diversity of the F6 generation and wild populations was not significantly different after the Kruskal–Wallis tests. However, the number of private alleles was lower in generation F6 (0.80) than in the overall average for wild populations (1.28). Discriminant analysis of principal components (DAPCs) supported analysis of molecular variance (AMOVA) by showing that there were no significant overlaps of inertia ellipses among F6 generation and wild populations. The experimental population differentiated into a separate group because of the random brood stock contribution from only a few fish from the wild population. The results suggest that the initial genetic diversity of the brood stock was sufficient as shown by the high number of alleles and polymorphic information content (PIC) values which contributed to the success of the selection program. The selection program successfully produced large fish at harvest and ensured genetic diversity of the F6 generation.</p>\n </div>","PeriodicalId":8104,"journal":{"name":"Aquaculture Research","volume":"2024 1","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/are/4440384","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Research","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/are/4440384","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Aquaculture in sub-Saharan African accounts for less than 2% of world aquaculture production. The use of unimproved species limits the growth of aquaculture. In order to increase production, there is a need to develop aquaculture species that show vigor and adapt to different culture conditions. A genetic improvement program for Oreochromis shiranus was initiated at the National Aquaculture Center (NAC), in Zomba, Malawi. The program used pedigree records during mate allocation to avoid mating of siblings. After six consecutive generations of selection, a sample of 32 fish was taken for genetic analysis using a panel of 16 microsatellite markers. Genetic diversity and population differentiation indices of the sixth filial generation (F6) were compared with wild fish populations sampled from the corresponding locations from which the initial brood stock was collected. The study results show that the genetic diversity of the F6 generation and wild populations was not significantly different after the Kruskal–Wallis tests. However, the number of private alleles was lower in generation F6 (0.80) than in the overall average for wild populations (1.28). Discriminant analysis of principal components (DAPCs) supported analysis of molecular variance (AMOVA) by showing that there were no significant overlaps of inertia ellipses among F6 generation and wild populations. The experimental population differentiated into a separate group because of the random brood stock contribution from only a few fish from the wild population. The results suggest that the initial genetic diversity of the brood stock was sufficient as shown by the high number of alleles and polymorphic information content (PIC) values which contributed to the success of the selection program. The selection program successfully produced large fish at harvest and ensured genetic diversity of the F6 generation.

Abstract Image

查看原文本刊更多论文

六代选育后shiranus Oreochromis （Boulenger 1905）与相应野生群体的遗传多样性比较

撒哈拉以南非洲的水产养殖占世界水产养殖产量的比例不到2%。未经改良品种的使用限制了水产养殖业的发展。为了提高产量，需要发展具有活力和适应不同养殖条件的养殖品种。马拉维Zomba国家水产养殖中心（NAC）启动了一项shiranus Oreochromis遗传改良计划。该程序在配偶分配时使用系谱记录，以避免兄弟姐妹交配。经过连续6代的选择，采用16个微卫星标记对32条鱼进行遗传分析。将子代第六代（F6）的遗传多样性和种群分化指数与原始育雏种群所在地点的野生种群进行了比较。研究结果表明，经Kruskal-Wallis检验，F6代与野生居群的遗传多样性无显著差异。然而，F6代的私有等位基因数量（0.80个）低于野生群体的总体平均水平（1.28个）。主成分（DAPCs）判别分析支持分子方差分析（AMOVA），表明F6代与野生居群的惯性椭圆不存在显著重叠。实验种群分化为一个单独的群体，因为只有来自野生种群的少数鱼的随机种群贡献。结果表明，该育雏群体的初始遗传多样性是充分的，等位基因数量和多态性信息含量（PIC）值较高，这是选择方案成功的原因之一。该选择方案成功地在收获时产生了大型鱼，并确保了F6代的遗传多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture Research 农林科学-渔业

CiteScore

4.60

自引率

5.00%

发文量

464

审稿时长

5.3 months

期刊介绍： International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.