Phytochemical profiling of Prunus armeniaca kernel extracts and exploration of their multifaceted antioxidant and antibacterial effects through in vitro and in silico studies

IF 1.9 4区 医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE
Mohamed Amine El-Hajjaji , Kawtar Fikri-Benbrahim , Mohammed El Ouassete , Nidal Naceiri Mrabti , Najoua Soulo , Asmae El Ghouizi , Badiaa Lyoussi , Zineb Benziane Ouaritini
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引用次数: 0

Abstract

Introduction

Prunus armeniaca "Apricot" kernels are considered a by-product widely employed in traditional medicine for treating various ailments due to their potential therapeutic properties. The current investigation sought to assess the properties of aqueous and methanol extracts of Prunus armeniaca kernels (PAK).

Methods

The phytochemical composition of the studied extracts was evaluated using high-performance liquid chromatography with a diode array detector (HPLC-DAD). Total antioxidant capacity (TAC), β-carotene bleaching, reducing power (RP), and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were used to assess antioxidant activity. Using the disc diffusion method and the microdilution test in 96-well microplates against four bacterial strains, the antimicrobial activity of the aforementioned extracts was evaluated. The potential of several phenolic compounds as bacterial inhibitors was assessed through extensive density functional theory (DFT) calculations and molecular docking analyses.

Results

The phytochemical analysis revealed the identification of several phenolic constituents, with tocopherol (11.61 ± 0.9 mg/g of dry extract) and resveratrol (25.3 ± 0.4 mg/g of dry extract) emerging as the major compounds in the aqueous and methanolic extracts, respectively. Both extracts exhibited remarkable antioxidant activity with IC₅₀ values of 1.47 ± 0.08 and 1.97 ± 0.15 mg/mL (DPPH), EC₅₀ = 3.682 ± 0.123 and 12.415 ± 0.344 mg/mL (RP), TAC values of 6.486 ± 0.275 and 5.323 ± 0.826 mg AAE/g, and β-carotene values of 56 ± 0.553 % and 50 ± 0.645 % respectively. Moreover, the aqueous extract showed the highest antibacterial activity against Staphylococcus aureus with a MIC value of 0.625 mg/mL, and an inhibition zone diameter of 11.5 ± 0.71 mm. The combined analysis of docking results and DFT calculations for various phenolic compounds against Staphylococcus aureus (Glycyl-penicillin-binding protein 2a) and Escherichia coli (DNA Gyrase B) revealed significant interactions and reactivities.

Conclusion

These results clearly indicate that PAK contains a high concentration of bioactive compounds, especially tocopherol and resveratrol, and may serve in the treatment of various diseases thanks to its extracts' promising antioxidant and antimicrobial activities.
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来源期刊
European Journal of Integrative Medicine
European Journal of Integrative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
4.70
自引率
4.00%
发文量
102
审稿时长
33 days
期刊介绍: The European Journal of Integrative Medicine (EuJIM) considers manuscripts from a wide range of complementary and integrative health care disciplines, with a particular focus on whole systems approaches, public health, self management and traditional medical systems. The journal strives to connect conventional medicine and evidence based complementary medicine. We encourage submissions reporting research with relevance for integrative clinical practice and interprofessional education. EuJIM aims to be of interest to both conventional and integrative audiences, including healthcare practitioners, researchers, health care organisations, educationalists, and all those who seek objective and critical information on integrative medicine. To achieve this aim EuJIM provides an innovative international and interdisciplinary platform linking researchers and clinicians. The journal focuses primarily on original research articles including systematic reviews, randomized controlled trials, other clinical studies, qualitative, observational and epidemiological studies. In addition we welcome short reviews, opinion articles and contributions relating to health services and policy, health economics and psychology.
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