Kátia Andrea de Menezes Torres , Sônia Maria Rolim Rosa Lima , Maria Thereza Gamberini , Domingos Sávio Rodrigues , Pedro Ismael Silva Junior
{"title":"Group B Strep During Pregnancy and Allium sativum L.","authors":"Kátia Andrea de Menezes Torres , Sônia Maria Rolim Rosa Lima , Maria Thereza Gamberini , Domingos Sávio Rodrigues , Pedro Ismael Silva Junior","doi":"10.1016/j.hermed.2024.100965","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Maternal colonisation with <em>Streptococcus agalactiae</em>, or Group B <em>Streptococcus</em> (<strong>GBS</strong>), during pregnancy increases the risk of neonatal infection through vertical transmission from the mother to the foetus, either before or during labour. This study aimed to evaluate the antimicrobial activity of the Sep-Pak 80% (<strong>SP80</strong>) fraction derived from <em>Allium sativum</em> L. and its synergistic potential with antibiotics against GBS strains.</div></div><div><h3>Methods</h3><div>Antimicrobial activity and potential synergism were assessed via broth microdilution and disk diffusion assays. Fifty-five clinical isolates and one American type culture collection (ATCC) strain of GBS were tested with the disk diffusion method to assess the combination of the SP80 fraction with ampicillin and penicillin G. A broth microdilution assay was performed on one ATCC strain.</div></div><div><h3>Results</h3><div>The minimum inhibitory concentrations of SP80, ampicillin, and penicillin G against <em>Streptococcus agalactiae</em> (ATCC 12386) were 5 mg/mL, 0.01 µg/mL, and 0.06 µg/mL, respectively. The combination of SP80 with antibiotics, assessed through broth microdilution, demonstrated an additive effect. Statistical analysis revealed that the mean inhibition zone for ampicillin, when combined with the SP80 fraction using the disk diffusion method, was greater than that for ampicillin alone, although the difference was not statistically significant. For penicillin G, the mean remained unchanged when it was combined with SP80. The inhibition zones of the isolated antibiotics revealed that the tested strains were more resistant to penicillin G than to ampicillin.</div></div><div><h3>Conclusions</h3><div>The SP80 fraction exhibits antimicrobial activity against GBS. When combined with antibiotics, it has an additive effect, suggesting that this approach could be promising for combating GBS infections.</div></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"48 ","pages":"Article 100965"},"PeriodicalIF":2.2000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herbal Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210803324001222","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction
Maternal colonisation with Streptococcus agalactiae, or Group B Streptococcus (GBS), during pregnancy increases the risk of neonatal infection through vertical transmission from the mother to the foetus, either before or during labour. This study aimed to evaluate the antimicrobial activity of the Sep-Pak 80% (SP80) fraction derived from Allium sativum L. and its synergistic potential with antibiotics against GBS strains.
Methods
Antimicrobial activity and potential synergism were assessed via broth microdilution and disk diffusion assays. Fifty-five clinical isolates and one American type culture collection (ATCC) strain of GBS were tested with the disk diffusion method to assess the combination of the SP80 fraction with ampicillin and penicillin G. A broth microdilution assay was performed on one ATCC strain.
Results
The minimum inhibitory concentrations of SP80, ampicillin, and penicillin G against Streptococcus agalactiae (ATCC 12386) were 5 mg/mL, 0.01 µg/mL, and 0.06 µg/mL, respectively. The combination of SP80 with antibiotics, assessed through broth microdilution, demonstrated an additive effect. Statistical analysis revealed that the mean inhibition zone for ampicillin, when combined with the SP80 fraction using the disk diffusion method, was greater than that for ampicillin alone, although the difference was not statistically significant. For penicillin G, the mean remained unchanged when it was combined with SP80. The inhibition zones of the isolated antibiotics revealed that the tested strains were more resistant to penicillin G than to ampicillin.
Conclusions
The SP80 fraction exhibits antimicrobial activity against GBS. When combined with antibiotics, it has an additive effect, suggesting that this approach could be promising for combating GBS infections.
期刊介绍:
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.