Lipid profiles and DNA barcoding markers reveal the genetic delimitation between species of the Carapa genus native from the Amazon

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2024-11-28 DOI:10.1016/j.bse.2024.104923

José Francisco de Carvalho Gonçalves , Gleisson de Oliveira Nascimento , Josiane Celerino de Carvalho , Alberdan Silva Santos , Doriane Picanço Rodrigues , Thais Andrade Germano , Mário Silva Nunes , Diego Pereira Souza , João Henrique F. Cavalcanti , Márcio Viana Ramos , Luis Felipe Ventorim Ferrão , Karina Martins

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引用次数: 0

Abstract

Authentic identification of Amazonian tree species is important for conservation and their sustainable exploitation. This work aimed to characterize the inter- and intraspecific divergence in two taxonomic units of the genus Carapa using the lipidomic profile of the seed oils, analyzed by gas chromatography-mass spectrometry (GC-MS) and DNA barcoding molecular markers to identify and discriminate differences between Carapa species. Seeds and leaves of 53 individuals were collected and morphologically grouped into Carapa guianensis and Carapa vasquezii. The oils extracted from seeds were esterified, and their lipidomic profiles were analyzed. Two chloroplast genome regions (psbA-trnH and rbcL) were amplified for genetic diversity analyses and the barcoding-gap test. The lipidomic profile allowed the identification of 40 substances present in the C. guianensis and C. vasquezii oils, with 20 shared among the species independent of the region of collection, two occurring only in the oil of C. guianensis from Acre, four in samples of C. guianensis and C. vasquezii from Amazonas, and 16 substances occurring only in samples of C. vasquezii oil. C. vasquezii presented a greater diversity of terpenoids. The genome regions made it possible to obtain 16 distinct haplotypes for the two species (C. guianensis and C. vasquezii), 999 conserved sites, and 32 polymorphic sites. The species presented significant molecular divergence (F_ST = 0.87), and the rbcL region was considered promising for barcode studies at levels ranging from 0.1 to 0.2% of genetic distance. The results suggest that the two species morphologically identified C. guianensis and C. vasquezii constitute genetically divergent biological groups.

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.