Characterization and regulatory analysis of betaine homocysteine S-methyltransferase gene 1 (BHMT1) in mud crab: A gene responsive to salinity and feeding behavior

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-11-28 DOI:10.1016/j.genrep.2024.102102

Jinju Yin , Zhiqiang Liu , Xin Jin , Wei Wang , Lingbo Ma , Ming Zhao

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引用次数: 0

Abstract

Betaine homocysteine S-methyltransferase gene 1 (BHMT1) encoded betaine homocysteine S-methyltransferase (BHMT) catalyzes the homocysteine-to-methionine reaction using betaine as a methyl donor. Previously, we found that BHMT was lost in the genome of the insect clade of arthropods, but was highly expressed in the mandibular organ (MO) of the mud crab Scylla paramamosain (Sp). To further explore its significance, we performed a primary regulatory analysis of BHMT in mud crabs. The open reading frame (ORF) length of Sp-BHMT1 was 1203 bp, encoding 400 amino acids. Sequence alignment revealed that BHMT1 was highly conserved in different animals, and its identity was higher in more closely related species. Sp-BHMT1 had the highest expression in the MO of both sexes, while its expression in the MO was 1.6-fold higher in males than in females; similar results were also found in the cerebral ganglion, hepatopancreas, and thoracic ganglia tissues. During larval development, Sp-BHMT1 was weakly expressed on most days but was significantly elevated on the first day of the Zoea 2nd (Z2) and Z3 stages. Sp-BHMT1 exhibited the highest expression at a salinity of 10 ‰ and the lowest at a salinity of 30 ‰. With decreasing salinity, the expression of Sp-BHMT1 increased significantly at 6 h and then returned to baseline at 8 h. After starvation treatment, the expression of Sp-BHMT1 was significantly upregulated compared to that in the feed group at all examined time points, with a peak expression at 18:00 on the third day in the starvation group. Krüppel homolog 1 (Kr-h1), a methyl farnesoate (MF) signal gene, could increase Sp-BHMT1 expression under low salinity and starvation, suggesting that a “salinity/starvation→BHMT1 → MF biosynthesis” regulatory axis might exist in crabs. This study provides valuable insights into the functional study of Sp-BHMT1 and MF biosynthetic regulation.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.