Growth, fat metabolism and hepatic health in largemouth bass fed varying fat-level diets

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-11-30 DOI:10.1016/j.aqrep.2024.102535

Digen Qin , Jiale Chen , Junxiong Li , Zhiyou Liu , Weibin Huang , Yuanzhi Yang , Jing Zhang , Beiping Tan , Xiaohui Dong

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Abstract

This 60-day experiment investigated the influences of growth, fat metabolism and hepatic health of largemouth bass (Micropterus salmoides) fed varying fat diets. 225 fish (34 ± 0.04 g) were divided into three groups and fed diets with fat levels of 10 % (Low-fat diet, LFD), 14 % (Medium-fat diet, MFD) and 18 % (High-fat diet, HFD), respectively. The results revealed that fish fed HFD exhibited significantly better protein efficiency ratio (PER) and lower feed conversion ratio (FCR) than LFD group (P<0.05), but survival rate (SR) and weight gain rate (WGR) were unaffected (P>0.05). Dietary fat levels significantly decreased hepatosomatic index (HSI) of fish (P<0.05), but visceral somatic index (VSI) and condition factor (CF) were unaffected (P>0.05). The contents of fat and ash in whole-body, and hepatic fat contents were increased with dietary fat levels (P<0.05), the contents of moisture and crude protein in whole-body and liver exhibited no substantial variations (P>0.05). Serum biochemical indexes and serum alanine aminotransferase (ALT) activity, liver glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased significantly with rising dietary fat levels (P<0.05), serum aspartate transaminase (AST) and liver superoxide dismutase (SOD) activities had no statistical differences (P>0.05). The expression of srebp-1 and acc in liver were down-regulated, but atgl, aco and bax were up-regulated with rising dietary fat contents (P<0.05). Caspase-3 exhibited a remarkable tendency of initially rising and then declining (P<0.05), the expression of fas, cpt-I, bcl-2 and the ratio of bcl-2/bax were unaffected (P>0.05). The histology of liver indicated the size and quantity of fat droplets increased with increment of dietary fat intake. Conclusion: the dietary fat levels of 14 %-18 % could be conducive to better growth of largemouth bass in this study.

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不同脂肪水平饲料对大口黑鲈生长、脂肪代谢和肝脏健康的影响

本试验旨在研究不同脂肪饲料对大口黑鲈生长、脂肪代谢和肝脏健康的影响。225尾鱼（34 ± 0.04 g）随机分为3组，分别饲喂脂肪水平为10 %（低脂饲料，LFD）、14 %（中脂饲料，MFD）和18 %（高脂饲料，HFD）的饲料。结果表明：与LFD组相比，HFD组鱼的蛋白质效率（PER）显著提高，饲料系数（FCR）显著降低（P>0.05），但成活率（SR）和增重率（WGR）未受影响（P>0.05）。饲料脂肪水平显著降低了鱼的肝体指数（HSI）（P>0.05），但内脏体指数（VSI）和条件因子（CF）未受影响（P>0.05）。全鱼脂肪、灰分含量和肝脏脂肪含量随饲粮脂肪水平的升高而升高（P>0.05），全鱼和肝脏水分和粗蛋白质含量无显著变化（P>0.05）。血清生化指标及血清谷丙转氨酶（ALT）活性、肝脏谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）活性随着饲粮脂肪水平的升高而显著升高（P>0.05），血清天冬氨酸转氨酶（AST）和肝脏超氧化物歧化酶（SOD）活性差异无统计学意义（P>0.05）。随着饲料脂肪含量的升高，肝脏中srebp-1和acc的表达下调，atgl、aco和bax的表达上调（P<0.05）。Caspase-3表现出先上升后下降的显著趋势（P>0.05）， fas、cpt-I、bcl-2的表达及bcl-2/bax的比值未受影响（P>0.05）。肝脏组织学显示脂肪滴的大小和数量随饲料脂肪摄入量的增加而增加。结论：饲料脂肪水平为14 % ~ 18 %有利于大口黑鲈较好的生长。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.