Integrative transcriptomic and metabolomic reveals squalene alleviating the inflammatory response and enhancing immunity of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-11-30 DOI:10.1016/j.aqrep.2024.102524

Xiaobo Yan , Simiao Pan , Xiangxiang Suo , Weibin Huang , Tao Li , Shuang Zhang , Yuanzhi Yang , Beiping Tan , Xiaohui Dong

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引用次数: 0

Abstract

Our previous study on plant oil substitution for fish oil found that corn oil induced inflammatory responses in grouper, while olive oil was effective in anti-inflammatory and immune enhancing. As a vital active ingredient in olive oil, squalene has been widely studied for its antioxidant and anti-inflammatory properties. Therefore, the present study was designed to use corn oil as the main lipid source to create a model of inflammation or low immunity and investigate the regulatory effect of squalene on the immunity of grouper, and to perform hepatic transcriptomic and metabolomic assays of grouper using omics technology, with the aim of providing basic data for the study of the mechanism of squalene. The results showed: squalene ingestion did not affect the growth performance of grouper (P > 0.05) but significantly improved immunity and the resistance of grouper to Vibrio harveyi (P < 0.05). Transcriptomic and metabolomic analyses showed that squalene activated the Wnt/β-Catenin signaling pathway and PI3K-Akt signaling pathway. Western blot results showed that squalene ingestion significantly upregulated Wnt/β-Catenin protein expression and downregulated the Axin protein expression (P < 0.05). Above results demonstrated that squalene enhanced immunity and disease resistance in grouper may be related to the activation of PI3K-Akt/ Wnt/β-Catenin signaling pathway, while the activation of β-Catenin could reduce the inflammatory response by inhibiting NF-κB-mediated inflammatory factors. The findings will contribute to the application of squalene as an immune enhancer for aquaculture and medical industry.

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综合转录组学和代谢组学研究发现角鲨烯可减轻杂交石斑鱼（♀Epinephelus fuscoguttatus ×♂E. lanceolatu）的炎症反应和增强免疫力

我们之前对植物油替代鱼油的研究发现，玉米油可引起石斑鱼的炎症反应，而橄榄油具有有效的抗炎和免疫增强作用。作为橄榄油中重要的活性成分，角鲨烯因其抗氧化和抗炎特性而被广泛研究。因此，本研究拟以玉米油为主要脂质源，建立炎症或低免疫模型，研究角鲨烯对石斑鱼免疫的调节作用，并利用组学技术对石斑鱼进行肝脏转录组学和代谢组学分析，为研究角鲨烯的作用机制提供基础数据。结果表明：角鲨烯摄食不影响石斑鱼的生长性能（P <； 0.05），但显著提高了石斑鱼对哈维弧菌的免疫力和抵抗力（P <； 0.05）。转录组学和代谢组学分析表明，角鲨烯激活了Wnt/β-Catenin信号通路和PI3K-Akt信号通路。Western blot结果显示，摄入角鲨烯显著上调Wnt/β-Catenin蛋白表达，下调Axin蛋白表达（P <； 0.05）。以上结果表明，角鲨烯增强石斑鱼免疫和抗病能力可能与激活PI3K-Akt/ Wnt/β-Catenin信号通路有关，而激活β-Catenin可通过抑制NF-κ b介导的炎症因子来减轻炎症反应。该研究结果将有助于角鲨烯作为免疫增强剂在水产养殖和医疗行业的应用。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.