The effects of short-term non-weightbearing and immobilization after anterior cruciate ligament reconstruction on articular cartilage: Long-term observation after reloading and remobilization

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2024-11-22 DOI:10.1016/j.tice.2024.102628

Akinori Kaneguchi, Ryo Okahara, Nanami Masuhara, Yoshika Doi, Kaoru Yamaoka, Junya Ozawa

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引用次数: 0

Abstract

Non-weightbearing or immobilization after anterior cruciate ligament (ACL) reconstruction accelerates cartilage degeneration. However, it is unclear whether these adverse effects are reversed by reloading or remobilization. Moreover, it is unknown whether the combination of non-weightbearing and immobilization after ACL reconstruction has synergistic effects on cartilage degeneration. We aimed to determine 1) the long-term effects of reloading or remobilization following short-term non-weightbearing or immobilization after ACL reconstruction on cartilage degeneration and 2) the combined effects of non-weightbearing and immobilization on cartilage degeneration. We divided ACL-reconstructed rats into four groups: no intervention, non-weightbearing, joint immobilization, and non-weightbearing plus immobilization. Non-weightbearing and immobilization were performed for 2 weeks, after which all rats were reared without intervention. Untreated rats were used as controls. At 2, 4, or 12 weeks after starting the experiment, cartilage degeneration in the anterior, middle, and posterior regions of the medial tibial plateau was histologically assessed. Two weeks of non-weightbearing or immobilization after ACL reconstruction facilitated cartilage degeneration in the middle and posterior regions compared to those with no intervention. Cartilage degeneration was not reversed by 10 weeks of reloading or remobilization. Compared with non-weightbearing alone, combination of non-weightbearing and immobilization improved cartilage degeneration in the middle region, but worsened it in the posterior region. Cartilage degeneration induced by 2 weeks of non-weightbearing or immobilization after ACL reconstruction was not reversed by reloading or remobilization. Thus, to reduce cartilage degeneration, non-weightbearing and immobilization should be avoided after ACL reconstruction, even for short-term.

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前交叉韧带重建后短期不负重和固定对关节软骨的影响：重装和再活动后的长期观察

前交叉韧带（ACL）重建后不负重或固定加速软骨退变。然而，尚不清楚这些不利影响是否可以通过重新装填或再动员来逆转。此外，前交叉韧带重建后非负重和固定联合是否对软骨退变有协同作用尚不清楚。我们的目的是确定1)ACL重建后短期不负重或固定后重新加载或再活动对软骨退变的长期影响，以及2)不负重和固定对软骨退变的联合影响。我们将acl重建大鼠分为四组：不干预、不负重、关节固定和不负重加固定。非负重和固定2周后，所有大鼠在不干预的情况下饲养。以未治疗的大鼠为对照。实验开始后2周、4周或12周，对胫骨内侧平台前、中、后区域的软骨退变进行组织学评估。与未进行干预的患者相比，前交叉韧带重建后两周的非负重或固定治疗促进了中后部软骨退变。软骨退行性变在10周的重新装载或再固定后未得到逆转。与单纯不负重相比，不负重联合固定改善了中段软骨退变，但加重了后部软骨退变。前交叉韧带重建后2周不负重或固定引起的软骨退变不能通过重新加载或再固定逆转。因此，为了减少软骨退变，ACL重建后应避免非负重和固定，即使是短期的。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.