{"title":"Elucidation of the fine structure and anti–breast tumor activity of a glucomannan from the pseudobulbs of Pleione bulbocodioides","authors":"Wan-Qi Deng, Shao-Wei Han, Si-Yuan Shao, Shuai Li","doi":"10.1016/j.carbpol.2024.123062","DOIUrl":null,"url":null,"abstract":"<div><div>Pseudobulbs of <em>Pleione bulbocodioides</em> have been used as traditional Chinese medicine for a long time to treat cancers through decoction with water. However, as its main water-soluble ingredient, the structure of polysaccharide has not been elucidated yet. To obtain its polysaccharide with antitumor activity, a series of isolation, structural identification and antitumor evaluation experiments were performed. As a result, a newly discovered glucomannan, named PBP, was isolated and purified from the pseudobulbs of <em>P. bulbocodioides</em>. The molecular weight of PBP is 1.11 × 10<sup>5</sup> Da. Its monosaccharide composition comprises mannose and glucose at a molar ratio of 5:3. The fine structure of PBP was confirmed as a linear glucomannan backbone primarily comprising repeating →4)-β-<span>d</span>-Glc-(1 → 4)-β-<span>d</span>-Man-(1 → 4)-β-<span>d</span>-Glc-(1 → 4)-β-<span>d</span>-Man-(1 → 4)-β-<span>d</span>-Glc-(1 → 4)-β-<span>d</span>-Man-(1→4)-β-<span>d</span>-Man-(1 → 4)-β-<span>d</span>-Man-(1 → via a combination of analytical techniques and enzymolysis methods. PBP effectively inhibited the growth of 4T1 tumors with low toxicity in vivo<em>.</em> Furthermore, a noteworthy augmentation in the expression levels of CD4+ and CD8+ T cells suggested the existence of an elevated immune response. In summary, a new glucomannan with anti-breast tumor activity was obtained, which has the potential to contribute to breast cancer therapy.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"351 ","pages":"Article 123062"},"PeriodicalIF":10.7000,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Polymers","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0144861724012888","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
Pseudobulbs of Pleione bulbocodioides have been used as traditional Chinese medicine for a long time to treat cancers through decoction with water. However, as its main water-soluble ingredient, the structure of polysaccharide has not been elucidated yet. To obtain its polysaccharide with antitumor activity, a series of isolation, structural identification and antitumor evaluation experiments were performed. As a result, a newly discovered glucomannan, named PBP, was isolated and purified from the pseudobulbs of P. bulbocodioides. The molecular weight of PBP is 1.11 × 105 Da. Its monosaccharide composition comprises mannose and glucose at a molar ratio of 5:3. The fine structure of PBP was confirmed as a linear glucomannan backbone primarily comprising repeating →4)-β-d-Glc-(1 → 4)-β-d-Man-(1 → 4)-β-d-Glc-(1 → 4)-β-d-Man-(1 → 4)-β-d-Glc-(1 → 4)-β-d-Man-(1→4)-β-d-Man-(1 → 4)-β-d-Man-(1 → via a combination of analytical techniques and enzymolysis methods. PBP effectively inhibited the growth of 4T1 tumors with low toxicity in vivo. Furthermore, a noteworthy augmentation in the expression levels of CD4+ and CD8+ T cells suggested the existence of an elevated immune response. In summary, a new glucomannan with anti-breast tumor activity was obtained, which has the potential to contribute to breast cancer therapy.
期刊介绍:
Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience.
The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.