Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Synthetic and Systems Biotechnology Pub Date : 2024-11-19 DOI:10.1016/j.synbio.2024.11.004

Yanting Cao , Jianghua Li , Long Liu , Guocheng Du , Yanfeng Liu

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Abstract

Metabolic engineering-driven microbial cell factories have made great progress in the efficient bioproduction of biochemical and recombinant proteins. However, the low efficiency and robustness of microbial cell factories limit their industrial applications. Harnessing microbial heterogeneity contributes to solving this. In this review, the origins of microbial heterogeneity and its effects on biosynthesis are first summarized. Synthetic biology-driven tools and strategies that can be used to improve biosynthesis by increasing and reducing microbial heterogeneity are then systematically summarized. Next, novel single-cell technologies available for unraveling microbial heterogeneity and facilitating heterogeneity regulation are discussed. Furthermore, a combined workflow of increasing genetic heterogeneity in the strain-building step to help in screening highly productive strains - reducing heterogeneity in the production process to obtain highly robust strains (IHP-RHR) facilitated by single-cell technologies was proposed to obtain highly productive and robust strains by harnessing microbial heterogeneity. Finally, the prospects and future challenges are discussed.

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利用微生物异质性促进合成生物学催化的生物合成

代谢工程驱动的微生物细胞工厂在高效生产生化蛋白和重组蛋白方面取得了很大进展。然而，微生物细胞工厂的低效率和鲁棒性限制了它们的工业应用。利用微生物异质性有助于解决这一问题。本文首先综述了微生物异质性的来源及其对生物合成的影响。然后系统总结了合成生物学驱动的工具和策略，这些工具和策略可用于通过增加和减少微生物异质性来改善生物合成。接下来，新的单细胞技术可用于揭示微生物异质性和促进异质性调节进行了讨论。此外，我们还提出了在菌株构建步骤中增加遗传异质性以帮助筛选高产菌株-在单细胞技术的促进下减少生产过程中的异质性以获得高健壮菌株（IHP-RHR）的组合工作流程，以利用微生物异质性获得高产健壮菌株。最后，对未来的展望和面临的挑战进行了讨论。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

6.90

自引率

12.50%

发文量

审稿时长

67 days

期刊介绍： Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.