Taste preference and conditioned taste aversion of the metallothionein-1/2 null mice

IF 2.4 3区 医学 Q2 BEHAVIORAL SCIENCES
Nanae Yasuura , Tadashi Inui , Tomohiko Yoshizawa , Norio Sogawa , Yoshiaki Sato , Makoto Funahashi
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Abstract

To investigate the effects of metallothionein (MT)-1/2 gene knockout on the taste preference and the conditioned taste aversion (CTA), we performed an experiment comparing the behavior of wild type (WT) mice and MT-1/2 null (KO) mice. The taste preference ratio of saccharin solutions (0.1 or 0.2 %) was significantly greater in KO mice than WT mice. The preference ratio of salt and bitter solutions was not different between KO mice and WT mice. After feeding with a low zinc diet, the average saccharin preference ratio was not significantly different between WT and KO mice at any concentration of saccharin. Concentrations of zinc in plasma in KO mice was slightly higher than WT mice, but both were within the physiological range. When conditioning for saccharin using intraperitoneal injection of LiCl, a marked CTA was produced in both WT and KO mice. These results suggest that MT may be involved in the regulation of sweet taste preferences and indicate that the brain function that mediates sweet taste-induced CTA acquisition is maintained in KO mice. In addition, it was suggested that MT was not actively involved in the regulation of plasma zinc concentration in either mice fed a normal diet or mice fed a low zinc diet for a week. The relation between the malfunction of MT-1/2 and signal transduction of intracellular zinc remained to be determined.
金属硫蛋白1/2缺失小鼠的味觉偏好和条件性味觉厌恶
为了研究金属硫蛋白(MT)-1/2基因敲除对味觉偏好和条件性味觉厌恶(CTA)的影响,我们进行了野生型(WT)小鼠和MT-1/2缺失(KO)小鼠行为的比较实验。糖精溶液(0.1%或0.2%)对KO小鼠的味觉偏好率显著高于WT小鼠。KO小鼠和WT小鼠对盐、苦溶液的偏好比无显著差异。在低锌日粮喂养后,在任何浓度的糖精下,WT和KO小鼠的平均糖精偏好比均无显著差异。KO小鼠血浆锌浓度略高于WT小鼠,但均在生理范围内。当通过腹腔注射LiCl调节糖精时,WT和KO小鼠都产生了显著的CTA。这些结果表明,MT可能参与了甜味偏好的调节,并表明在KO小鼠中,介导甜味诱导的CTA获取的脑功能得以维持。此外,这表明MT对正常饮食和低锌饮食小鼠血浆锌浓度的调节均不积极参与。MT-1/2的功能障碍与细胞内锌的信号转导之间的关系尚不清楚。
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来源期刊
Physiology & Behavior
Physiology & Behavior 医学-行为科学
CiteScore
5.70
自引率
3.40%
发文量
274
审稿时长
47 days
期刊介绍: Physiology & Behavior is aimed at the causal physiological mechanisms of behavior and its modulation by environmental factors. The journal invites original reports in the broad area of behavioral and cognitive neuroscience, in which at least one variable is physiological and the primary emphasis and theoretical context are behavioral. The range of subjects includes behavioral neuroendocrinology, psychoneuroimmunology, learning and memory, ingestion, social behavior, and studies related to the mechanisms of psychopathology. Contemporary reviews and theoretical articles are welcomed and the Editors invite such proposals from interested authors.
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