Novel NIR fluorescent probe based on BODIPY for diagnosis and treatment evaluation of alcoholic liver disease via visualizing HClO fluctuation

Abstract

Alcoholic liver disease (ALD) is gradually becoming common due to the increasing number of drinkers worldwide, which is a serious threat to human physical and mental health. In the process of ALD, it is often accompanied by the occurrence of inflammation, which induce high expression of reactive oxygen species including HClO. In this work, we successfully fabricated a NIR fluorescent probe BDP-ENE-Fur-HClO for real-time imaging alcoholic liver disease via tracing HClO. The probe displayed good sensitivity and specificity, rapid recognition speed and NIR emitting (700 nm) for detection of HClO in vitro. Based on the remarkable performances, probe was capable of tracing endogenous/exogenous HClO in living cells without interference from other ROS as well as in ALD cell model. Additionally, probe could monitor the exogenous HClO in normal mice and high expression of HClO in the peritonitis mice, that accomplishing the diagnosis of inflammation. What’s more, one simulated hazardous drinking ALD mice model and simulated excessive drinking (a type of alcohol use disorder) ALD mice model were developed, probe could image the alcoholic liver injury of mice by monitoring the HClO fluctuation in ALD mice, which affording a valid instrument for the diagnosis of ALD. Ultimately, after hepatoprotective drug administrating to the models, probe could triumphantly evaluate the treatment effect of drug on ALD.