Unveiling a novel mechanism: Reduction of graphene oxide by Lysinibacillus sp. through secretion of l-ascorbic acid

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere Pub Date : 2024-12-01 DOI:10.1016/j.chemosphere.2024.143813

Yuanyuan Zhou , Ziyi Lin , Yonghe Han , Li Gan , Ying Cheng , Zuliang Chen

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Abstract

The graphene oxide (GO) reduction by microorganisms has garnered considerable interest, yet the specific mechanisms underlying the bacteria secretion of reducing substances for GO reduction remain unclear. This study aims to learn that bacterial extracellular components can reduce graphene oxide through direct (contacting GO) and indirect (not contacting GO) reduction experiments. The subsequent investigation focused on identifying the specific substances secreted by bacteria capable of GO reduction. The results of non-targeted metabolomics revealed differential expression of cacid (L-AA) demonstrates a significant up-regulation. The further experiment involved the supplementation of L-AA in the reduction system of Lysinibacillus sp. with GO, demonstrating enhanced reduction efficacy, with the I_D/I_G ratio of reduced graphene oxide (rGO) increasing to 1.073 after 4 d of reduction with 0.5 g L⁻¹ L-AA. Therefore, the mediation of GO reduction by L-AA secreted by Lysinibacillus sp. is proposed as a viable mechanism, offering novel insights into microbial GO reduction.

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揭示一种新机制：赖氨酸杆菌通过分泌 L-抗坏血酸还原氧化石墨烯。

微生物对氧化石墨烯（GO）的还原引起了广泛关注，但细菌分泌还原物质还原 GO 的具体机制仍不清楚。本研究旨在通过直接（接触氧化石墨烯）和间接（不接触氧化石墨烯）还原实验了解细菌胞外成分可还原氧化石墨烯。随后的调查重点是确定细菌分泌的能够还原 GO 的特定物质。非靶向代谢组学的结果显示，酸（L-AA）的差异表达显示出显著的上调。进一步的实验是在赖氨酸杆菌的 GO 还原系统中补充 L-AA，结果表明还原效果增强，在使用 0.5 g L-1 L-AA 还原 4 d 后，还原氧化石墨烯（rGO）的 ID/IG 比值增至 1.073。因此，莱氏拟杆菌分泌的 L-AA 对 GO 还原的中介作用被认为是一种可行的机制，为微生物还原 GO 提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemosphere 环境科学-环境科学

CiteScore

15.80

自引率

8.00%

发文量

4975

审稿时长

3.4 months

期刊介绍： Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.