Protocol for fabricating elastomeric stencils for patterned stem cell differentiation.

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS
STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-11-26 DOI:10.1016/j.xpro.2024.103187
Stefanie Lehr, Jack Merrin, Monika Kulig, Thomas George Minchington, Anna Kicheva
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引用次数: 0

Abstract

Geometrically controlled stem cell differentiation promotes reproducible pattern formation. Here, we present a protocol to fabricate elastomeric stencils for patterned stem cell differentiation. We describe procedures for using photolithography to produce molds, followed by molding polydimethylsiloxane (PDMS) to obtain stencils with through holes. We then provide instructions for culturing cells on stencils and, finally, removing stencils to allow colony growth and cell migration. This approach yields reproducible two-dimensional organoids tailored for quantitative studies of growth and pattern formation. For complete details on the use and execution of this protocol, please refer to Lehr et al.1.

用于干细胞模式化分化的弹性模板制造规程。
几何控制的干细胞分化可促进可重现的图案形成。在此,我们介绍了一种用于干细胞模式分化的弹性模板制作方法。我们介绍了使用光刻技术制作模具的程序,然后模塑聚二甲基硅氧烷(PDMS)以获得带有通孔的模板。然后,我们介绍了在模板上培养细胞的方法,最后,我们还介绍了如何移除模板以实现集落生长和细胞迁移。这种方法能产生可重复的二维有机体,适合对生长和模式形成进行定量研究。有关使用和执行该方案的完整细节,请参阅 Lehr 等人的文章1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
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