Organophosphate and carbamate susceptibility profiling of Anopheles gambiae sl. across different ecosystems in southern Benin.

Q1 Medicine

Wellcome Open Research Pub Date : 2024-11-14 eCollection Date: 2024-01-01 DOI:10.12688/wellcomeopenres.21452.2

Camille Dossou, Genevieve Tchigossou, Massioudou Koto, Seun Michael Atoyebi, Eric Tossou, Danahé Adanzounon, Sandra Ateutchia Ngouanet, Haziz Sina, Innocent Djègbè, Adam Gbankoto, Charles Wondji, Rousseau Djouaka

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Abstract

Background: To overcome the spread of high pyrethroid resistance in the main malaria vectors and malaria disease persistence, it is crucial to look for effective and better resistance management strategies. Understanding the phenotypic profile of Anopheles gambiae sl. against alternatives insecticides like organophosphates and carbamates is crucial.

Methods: Anopheles larvae and pupae were collected from the breeding sites in rice fields, pineapple crop areas, and peri-urban areas. WHO susceptibility tests were conducted on unfed female mosquitoes aged 3-5 days old. Mosquitoes were exposed to malathion 5%, pirimiphos-methyl 0.25%, and bendiocarb 0.1% using the standard WHO protocol. Polymerase chain reaction (PCR) techniques were used to detect species, kdr and Ace-1 mutations.

Results: Anopheles gambiae sl. from Sèdjè-Dénou rice field population was resistant to bendiocarb (0.1%) with a mortality rate of 72.2% whereas Anopheles gambiae sl. populations from Zinvié-Dokomey (rice field), Zè-Tozounmè (pineapple field), and Adjagbo (peri-urban area) were suspected to be resistant with mortality rates of 90%, 93.5%, 95.4% respectively. However, all of them were susceptible to organophosphates (malathion and pirimiphos-methyl) with a mortality rate of 100%. PCR assay revealed that 100% of the mosquitoes tested were Anopheles coluzzii. The frequencies of Ace-1R mutation in all Anopheles coluzzii populations tested were low (3-27%).

Conclusions: Organophosphates (malathion and pirimiphos-methyl) have maintained their efficacy against Anopheles coluzzii populations from Sèdjè-Dénou (rice field), Zè Tozounmè (pineapple field), Zinvié Dokomey (rice field), or Adjagbo (peri-urban area). The good efficacy of these organophosphates against Anopheles coluzzii populations from the southern part of Benin are observed in the current study. The use of pirimiphos-methyl for IRS in this part of the country would be a successful alternative for malaria control in this area.

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贝宁南部不同生态系统中冈比亚按蚊对有机磷和氨基甲酸酯类药物的敏感性分析。

背景：为了克服拟除虫菊酯抗药性在主要疟疾病媒中的传播和疟疾疾病的持续存在，必须寻找有效和更好的抗药性管理策略。了解冈比亚按蚊对有机磷和氨基甲酸酯等替代杀虫剂的表型特征至关重要：方法：从稻田、菠萝种植区和城市周边地区的繁殖地收集冈比亚按蚊幼虫和蛹。对 3-5 天大的未喂食雌蚊进行了世界卫生组织药敏试验。按照世卫组织的标准方案，对蚊子进行马拉硫磷 5%、甲基吡啶磷 0.25% 和苯线磷 0.1% 的药敏试验。聚合酶链反应（PCR）技术用于检测物种、kdr和Ace-1突变：而来自 Zinvié-Dokomey（稻田）、Zè-Tozounmè（菠萝田）和 Adjagbo（城市周边地区）的冈比亚按蚊则被怀疑具有抗药性，死亡率分别为 90%、93.5% 和 95.4%。然而，它们都对有机磷（马拉硫磷和甲基吡啶磷）敏感，死亡率为 100%。聚合酶链式反应（PCR）检测显示，100% 的受检蚊子都是大肠按蚊（Anopheles coluzzii）。在所有接受测试的疟蚊种群中，Ace-1R 突变的频率都很低（3-27%）：结论：有机磷（马拉硫磷和甲基吡啶磷）对 Sèdjè-Dénou（稻田）、Zè Tozounmè（菠萝田）、Zinvié Dokomey（稻田）或 Adjagbo（城市周边地区）的大肠疟蚊种群保持了效力。本研究观察到，这些有机磷类杀虫剂对贝宁南部地区的 coluzzii 按蚊具有良好的杀虫效果。在贝宁的这一地区使用甲基吡啶磷进行室内滞留喷雾杀虫剂将是在这一地区成功控制疟疾的替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Wellcome Open Research Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

5.50

自引率

0.00%

发文量

426

审稿时长

1 weeks

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