Hydrogen peroxide priming triggers splicing memory in grape berries.

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Molecular Biology Pub Date : 2024-11-28 DOI:10.1007/s11103-024-01528-8

Ding-Ding Zuo, Hao-Ting Sun, Lu Yang, Meng-Ling Zheng, Jing Zhang, Da-Long Guo

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引用次数: 0

Abstract

Plants are highly sensitive to environmental changes, and alternative splicing (AS) has been described in many studies due to its important control role in stress response. Recent studies indicated that plants exhibit splicing memory to stress to effectively activate transcriptional adaptation. Hydrogen peroxide (H₂O₂), as a reactive oxygen species (ROS), has toxic effects on plants. However, it also has a significant effect on promoting early maturity of 'Kyoho' grape at low concentrations. To explore the mechanism of priming treatment of H₂O₂ showing better promotion effect, the RNA-Seq data of H₂O₂-primied and no-primied fruits were analyzed. The genes with H₂O₂ stress splicing memory were identified, accompanied by changes in H3K4me3 modification levels, and their splicing memory patterns were verified by PCR and agarose gel electrophoresis. This finding establishes a link between alternative splicing memory and fruit ripening under H₂O₂ regulation and contribute to develop the application of H₂O₂ in fruit ripening.

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过氧化氢引物触发葡萄浆果中的拼接记忆。

植物对环境变化高度敏感，而替代剪接（AS）因其在胁迫响应中的重要控制作用，已被许多研究描述。最近的研究表明，植物对胁迫表现出剪接记忆，以有效激活转录适应。过氧化氢（H2O2）作为一种活性氧（ROS），对植物有毒性作用。然而，在低浓度下，它对促进'Kyoho'葡萄的早熟也有显著效果。为了探索 H2O2 引物处理对葡萄早熟的促进作用，研究人员分析了 H2O2 引物处理和未引物处理葡萄果实的 RNA-Seq 数据。结果发现了具有 H2O2 胁迫剪接记忆的基因，这些基因伴随着 H3K4me3 修饰水平的变化，并通过 PCR 和琼脂糖凝胶电泳验证了它们的剪接记忆模式。这一发现建立了替代剪接记忆与 H2O2 调节下果实成熟之间的联系，有助于开发 H2O2 在果实成熟中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.