Roles of cancer histology type and HPV genotype in HPV ctDNA detection at baseline in cervical cancer: Implications for tumor burden assessment.

IF 3.5 4区 医学 Q3 CELL BIOLOGY
Pathobiology Pub Date : 2024-11-27 DOI:10.1159/000542638
Miseon Lee, Eun Ji Lee, Jun Kang, Keun Ho Lee, Sung Jong Lee, Sook Hee Hong, Hee Seung Kim, Ahwon Lee
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引用次数: 0

Abstract

Introduction: Human papillomavirus circulating tumor DNA (HPV ctDNA) is a promising biomarker for monitoring cervical cancer. HPV ctDNA level at baseline (before treatment) reflects tumor burden. However, reported HPV ctDNA detection rates at baseline have shown variations across studies, suggesting the existence of other potential contributing factors. This study aimed to identify additional factors that might influence HPV ctDNA detection at baseline, focusing on histology type and HPV genotypes (high-risk genotypes HPV16 and HPV18).

Methods: We retrospectively analysed blood samples at baseline prior to treatment from 92 patients diagnosed with HPV16- or HPV18-associated cervical cancer (FIGO IA2-IIIC2) between 2013 and 2020. HPV ctDNA was evaluated using digital droplet PCR (ddPCR).

Results: HPV ctDNA was detected at baseline in 41.3% of cases. Locally advanced cervical cancers had a higher (p = 0.028) detection rate at baseline than early stage cervical cancers. HPV ctDNA positivity was significantly (p = 0.048) higher for HPV18 (60%) than for HPV16 (34.3%). Adenocarcinoma/adenosquamous carcinoma had a higher HPV ctDNA detection rate at baseline (54.2%) than squamous cell carcinoma (36.8%), but not significantly (p = 0.212) higher.

Conclusion: This study found the impact of histology and HPV genotype on HPV ctDNA at baseline in cervical cancer. HPV18 and adenocarcinoma were associated with a higher baseline HPV ctDNA detection rate. These results suggest the need for different HPV ctDNA approaches for analyzing tumor burden. This finding may also serve as a useful reference for post-treatment surveillance studies.

宫颈癌基线 HPV ctDNA 检测中癌症组织学类型和 HPV 基因型的作用:对肿瘤负担评估的影响。
导言:人乳头瘤病毒循环肿瘤 DNA(HPV ctDNA)是一种很有前景的宫颈癌监测生物标记物。基线(治疗前)HPV ctDNA水平反映了肿瘤负荷。然而,不同研究报告的 HPV ctDNA 基线检出率存在差异,这表明还存在其他潜在的影响因素。本研究旨在找出可能影响基线HPV ctDNA检测的其他因素,重点关注组织学类型和HPV基因型(高危基因型HPV16和HPV18):我们回顾性分析了2013年至2020年期间诊断为HPV16或HPV18相关宫颈癌(FIGO IA2-IIIC2)的92名患者治疗前的基线血液样本。采用数字液滴 PCR(ddPCR)对 HPV ctDNA 进行了评估:结果:41.3%的病例在基线时检测到了 HPV ctDNA。与早期宫颈癌相比,局部晚期宫颈癌的基线检出率更高(p = 0.028)。HPV ctDNA 阳性率方面,HPV18(60%)明显高于 HPV16(34.3%)(p = 0.048)。腺癌/腺鳞癌的基线 HPV ctDNA 检出率(54.2%)高于鳞状细胞癌(36.8%),但并不明显(p = 0.212):本研究发现了组织学和 HPV 基因型对宫颈癌基线 HPV ctDNA 的影响。HPV18和腺癌与较高的基线HPV ctDNA检出率有关。这些结果表明,需要采用不同的 HPV ctDNA 方法来分析肿瘤负荷。这一发现也可作为治疗后监测研究的有用参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Pathobiology
Pathobiology 医学-病理学
CiteScore
8.50
自引率
0.00%
发文量
47
审稿时长
>12 weeks
期刊介绍: ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.
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