Isolation, identification, and potentiality of gut-derived probiotic bacteria from Heteropneustes fossilis, stinging catfish.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Advanced Veterinary and Animal Research Pub Date : 2024-09-29 eCollection Date: 2024-09-01 DOI:10.5455/javar.2024.k806
Abdul Kader Jilani, Md Nurul Haider, Abir Hasan, Md Adil Mahfuz, Md Nazmul Islam Rifat, Md Mubarack Hossain, Muhammad Mehedi Hasan
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引用次数: 0

Abstract

Objective: This study was conducted to isolate and identify probiotic bacteria from wild stinging catfish (Heteropneustes fossilis), a very popular high-valued aquaculture species of Bangladesh. The isolates were identified through conventional culture-based and molecular techniques.

Materials and methods: Stinging catfish harvested from natural sources of three sampling sites under two districts (Kishoreganj and Netrakona) were collected, dissected for gut content, and cultured onto Lactobacillus MRS Agar plates. Out of 60 bacterial isolates obtained, 10 were chosen for an in vitro evaluation of their probiotic potentials through pH and bile tolerance tests. The 16S rRNA gene sequences of the selected isolates were searched against the NCBI database using the Basic Local Alignment Search Tool for Nucleotides (BLASTNs).

Results: The isolates were identified as Staphylococcus arlettae, Bacillus subtilis, Staphylococcus succinus, Bacillus velezensis, Kocuria subflava, Macrococcus caseolyticus, Lysinibacillus sphaericus, Glutamicibacter mysorens, Bacillus cereus, and Acinetobacter lwoffii. Among them, B. subtilis, S. succinus, B. velezensis, M. caseolyticus, G. mysorens, and B. cereus exhibited notable growth across all tested pH levels (pH 2, 3, and 4) and bile salt concentrations (0.3%, 0.5%, and 1.0%) suggesting that they have strong potential as probiotic bacteria. In addition, S. arlettae also indicated promising growth except at pH 2. L. sphaericus and K. subflava exhibited limited growth at low pH but tolerated bile salt concentrations. A. lwoffii did not show any growth at pH tests but minimal growth at the lower concentrations of bile salts.

Conclusion: According to the potentiality assessments and previous literature reviews, five isolates such as B. subtilis, S. succinus, M. caseolyticus, G. mysorens, and B. cereus were identified as potential probiotic bacteria. As species-specific probiotics are considered to perform more effectively and efficiently than unknown-sourced commercial probiotics, the findings of this study will be applicable in enhancing the aquaculture production of stinging catfish.

刺鲶肠道益生菌的分离、鉴定和潜力。
研究目的本研究旨在从野生刺鲶(Heteropneustes fossilis)中分离和鉴定益生菌,刺鲶是孟加拉国非常受欢迎的高价值水产养殖品种。分离的益生菌通过传统的培养和分子技术进行鉴定:从两个地区(Kishoreganj 和 Netrakona)的三个采样点的自然水域中采集刺鲶,剖检肠道内容物,并在乳酸杆菌 MRS 琼脂平板上培养。在获得的 60 个细菌分离物中,选择了 10 个通过 pH 值和胆汁耐受性测试对其益生菌潜力进行体外评估。使用核苷酸基本局部比对搜索工具(BLASTNs)在 NCBI 数据库中搜索了所选分离物的 16S rRNA 基因序列:结果:所分离的菌株被鉴定为 arlettae 葡萄球菌、枯草芽孢杆菌、琥珀葡萄球菌、韦列兹芽孢杆菌、亚弗拉瓦球菌、酪酸大球菌、来苏芽孢杆菌、米氏谷氨酸杆菌、蜡样芽孢杆菌和卢弗氏不动杆菌。其中,B. subtilis、S. succinus、B. velezensis、M. caseolyticus、G. mysorens 和 B. cereus 在所有测试的 pH 值(pH 值 2、3 和 4)和胆盐浓度(0.3%、0.5% 和 1.0%)下都表现出显著的生长,表明它们具有作为益生菌的强大潜力。L. sphaericus 和 K. subflava 在低 pH 值条件下生长有限,但能耐受胆盐浓度。A. lwoffii 在 pH 值测试中没有表现出任何生长,但在较低浓度的胆汁盐中有极小的生长:根据潜力评估和先前的文献综述,5 个分离菌株,如枯草芽孢杆菌、琥珀酰酵母菌、酪酸酵母菌、麦芽糖酵母菌和蜡样芽孢杆菌被确定为潜在的益生菌。由于物种特异性益生菌被认为比来源不明的商业益生菌更有效,本研究的结果将适用于提高刺鲶的养殖产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.70
自引率
7.10%
发文量
41
审稿时长
6 weeks
期刊介绍: Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.
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