Collagen properties of Indonesian local sheepskin isolated using acid and enzymatic methods.

IF 1.5 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Advanced Veterinary and Animal Research Pub Date : 2024-09-29 eCollection Date: 2024-09-01 DOI:10.5455/javar.2024.k823

Dita Prameswari Trenggono Putri, Vernanda Widya Pangestika, Hanifan Ilyas, Mohammad Zainal Abidin, Nanung Agus Fitriyanto, Yuny Erwanto

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引用次数: 0

Abstract

Objectives: This study aimed to investigate the physical properties of Indonesian local sheep skin collagen extracted by acid and enzymatic methods.

Materials and methods: Collagen was isolated from Pure Breed Garut Sheep (Ovis aries sp.) skin, 1.5 years old. The skins were obtained from a local slaughterhouse in Cirebon, Indonesia. The solvents used were CH₃COOH and three different enzymes: neutrase, alcalase, and bromelain.

Results: The highest yields of extracted collagen were bromelain-soluble collagen (BSC), which reached 37.07%. The range of Ph values for all samples started from 4.01 to 4.76. The viscosity values (cP) of acid-soluble collagen (ASC), neutrase-soluble collagen (NSC), alcalase-soluble collagen (LSC), and BSC were 3.42, 3.90, 3.45, and 3.12, respectively. Regarding SDS-PAGE analyses, Garut sheepskin collagen is categorized as collagen type I, which has a molecular weight of about 140.99 to 148.74 kDa for α1 and around 110 to 111.86 kDa for α2. The results of FTIR and DSC analyses for all samples show the same motif with commercial collagen motifs based on the literature.

Conclusion: Garut sheep skin has the potential to be an alternative raw material source for producing collagen. Collagen extracted using a combination of CH3COOH and bromelain enzyme showed the most desirable results in almost every characteristic.

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用酸法和酶法分离印尼当地羊皮的胶原蛋白特性。

研究目的本研究旨在调查用酸法和酶法提取的印尼当地绵羊皮肤胶原蛋白的物理性质：胶原蛋白是从 1.5 岁的纯种加鲁特绵羊（Ovis aries sp.）皮中分离出来的。绵羊皮取自印度尼西亚井里汶当地的屠宰场。使用的溶剂是 CH3COOH 和三种不同的酶：中性酶、脂肪酶和菠萝蛋白酶：提取胶原蛋白产量最高的是溴菠萝蛋白酶可溶性胶原蛋白（BSC），达到 37.07%。所有样品的 Ph 值范围从 4.01 到 4.76 不等。酸溶性胶原蛋白（ASC）、中性酶溶性胶原蛋白（NSC）、炼糖酶溶性胶原蛋白（LSC）和 BSC 的粘度值（cP）分别为 3.42、3.90、3.45 和 3.12。在 SDS-PAGE 分析中，加鲁特羊皮胶原蛋白被归类为 I 型胶原蛋白，α1 的分子量约为 140.99 至 148.74 kDa，α2 的分子量约为 110 至 111.86 kDa。所有样品的傅立叶变换红外光谱和 DSC 分析结果表明，根据文献资料，它们与商业胶原蛋白图案相同：结论：加鲁特羊皮有潜力成为生产胶原蛋白的替代原料来源。使用 CH3COOH 和菠萝蛋白酶组合提取的胶原蛋白在几乎所有特性方面都显示出最理想的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Advanced Veterinary and Animal Research Veterinary-Veterinary (all)

CiteScore

2.70

自引率

7.10%

发文量

审稿时长

6 weeks

期刊介绍： Journal of Advanced Veterinary and Animal Research (JAVAR) - is an open access, international, peer-reviewed, quarterly, highly-indexed scientific journal publishing original research findings and reviews on all aspects of veterinary and animal sciences. Basic and applied researches on- - Anatomy & histology - Animal health economics - Animal nutrition - Animal reproduction - Animal science - Antimicrobial resistance (AMR) - Biochemistry - Biotechnology - Dairy science - Epidemiology - Food hygiene and technology - Genetics and breeding - Immunology - Microbiology - Parasitology - Pathology - Pharmacology & toxicology - Physiology - Poultry science - Preventive veterinary medicine - Public health - Surgery & obstetrics - Veterinary extension studies - Wildlife & aquatic medicine - Zoo animal medicine.